文献类型: 外文期刊
作者:
Zhang Ruili
1
;
Yu Qun
1
;
Shi Guangliang
1
;
Liu Rui
1
;
Zhang Weiqian
1
;
Zhao Xia
1
;
Li Guangxing
1
;
Ge Ming
1
;
作者机构: 1.Northeast Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China
2.Chinese Acad Agr Sci, Harbin Vet Res Inst, Harbin 150001, Peoples R China
关键词: Astragalus polysaccharide;Bursa of Fabricius;Innate immunity;Toll-like receptor 4
期刊名称:BMC VETERINARY RESEARCH ( 影响因子:2.741; 五年影响因子:2.955 )
ISSN: 1746-6148
年卷期: 2017 年 13 卷
页码:
收录情况: SCI
摘要: Background: The Toll-like receptor 4 (TLR4) pathway involves in the pathogen recognition and defense against infection in mammals. Considering that avian and mammalian TLR are differentially mediated, the action of a natural product on avian TLR4 pathway was unclear. High, medium and low doses of Astragalus polysaccharide (APS), were treated the chicken at 7-days-old age by gavage. The sIgA level in the intestinal fluid, the expression of chTLR4 mRNA/protein in bursa of Fabricius as well as the expression of downstream molecules of chTLR4 (chMyD88, chTRIF, chNF-kappa B, chIRF3, chIFN-beta and chTNF-alpha) were measured on alternate days. Results: The content of sIgA and the chTLR4 mRNA expression/protein level were increased in non-dose-dependent manner after APS supplement. Also, the expressions of a subset of MyD88-independent pathway genes were more than MyD88-independent, in particular with low doses of APS supplement for 7 days. Conclusions: These suggest that administration of APS activates chTLR4 pathway in bursa of Fabricius in MyD88-independent pathway. Meanwhile, low dose of APS shows better performance regarding the activation of chTLR4 and regulation of MyD88-independent pathway.
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