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Immunogenicity and Protective Capacity of Sugar ABC Transporter Substrate-Binding Protein against Streptococcus suis Serotype 2, 7 and 9 Infection in Mice

文献类型： 外文期刊

作者： Yan, Zujie 1 ; Pan, Ruyi 1 ; Zhang, Junjie 1 ; Sun, Jianhe 3 ; Ma, Xiaochun 1 ; Dong, Nihua 1 ; Yao, Xiaohui 1 ; Wei, Jianchao 1 ; Liu, Ke 1 ; Qiu, Yafeng 1 ; Sealey, Katie 4 ; Nichols, Hester 5 ; Jarvis, Michael A. 5 ; Upton, Mathew 6 ; Li, Xiangdong 6 ; Ma, Zhiyong 1 ; Liu, Juxiang 2 ; Li, Beibei 1 ;

作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

2.Hebei Agr Univ, Coll Vet Med, Baoding 071000, Peoples R China

3.Shanghai Jiao Tong Univ, Shanghai Key Lab Vet Biotechnol, Shanghai 200240, Peoples R China

4.Univ Bristol, Sch Cellular & Mol Med, Univ Walk, Bristol BS8 1TD, England

5.Vaccine Grp Ltd, Plymouth PL6 8BU, England

6.Univ Plymouth, Sch Biomed Sci, Plymouth PL4 8AA, England

7.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China

关键词： Streptococcus suis; subunit vaccine; sugar ABC transporter substrate-binding protein; serotypes; universal vaccine

期刊名称：VACCINES （ 影响因子：7.8； 五年影响因子：7.4 ）

ISSN：

年卷期： 2024 年 12 卷 5 期

页码：

收录情况： SCI

摘要： Background: Streptococcus suis (S. suis) is a Gram-positive bacterium that causes substantial disease in pigs. S. suis is also an emerging zoonoses in humans, primarily in Asia, through the consumption of undercooked pork and the handling of infected pig meat as well as carcasses. The complexity of S. suis epidemiology, characterized by the presence of multiple bacterial serotypes and strains with diverse sequence types, identifies a critical need for a universal vaccine with the ability to confer cross-protective immunity. Highly conserved immunogenic proteins are generally considered good candidate antigens for subunit universal vaccines. Methods: In this study, the cross-protection of the sugar ABC transporter substrate-binding protein (S-ABC), a surface-associated immunogenic protein of S. suis, was examined in mice for evaluation as a universal vaccine candidate. Results: S-ABC was shown to be highly conserved, with 97% amino acid sequence identity across 31 S. suis strains deposited in GenBank. Recombinantly expressed S-ABC (rS-ABC) was recognized via rabbit sera specific to S. suis serotype 2. The immunization of mice with rS-ABC induced antigen-specific antibody responses, as well as IFN-gamma and IL-4, in multiple organs, including the lungs. rS-ABC immunization conferred high (87.5% and 100%) protection against challenges with S. suis serotypes 2 and 9, demonstrating high cross-protection against these serotypes. Protection, albeit lower (50%), was also observed in mice challenged with S. suis serotype 7. Conclusions: These data identify S-ABC as a promising antigenic target within a universal subunit vaccine against S. suis.

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