A getah virus-like-particle vaccine provides complete protection from viremia and arthritis in wild-type mice
文献类型： 外文期刊
作者： Miao, Qiuhong 1 ; Nguyen, Wilson 2 ; Zhu, Jie 3 ; Liu, Guangqing 3 ; van Oers, Monique M. 1 ; Tang, Bing 2 ; Yan, Kexin 2 ; Larcher, Thibaut 4 ; Suhrbier, Andreas 2 ; Pijlman, Gorben P. 1 ;
作者机构： 1.Wageningen Univ & Res, Lab Virol, Wageningen, Netherlands
2.QIMR Berghofer Med Res Inst, Inflammat Biol Grp, Brisbane, Australia
3.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Beijing, Peoples R China
4.UMR0703 APEX, INRAE, Oniris, Nantes, France
5.Australian Infect Dis Res Ctr, GVN Ctr Excellence, Brisbane, Qld, Australia
关键词： Getah virus; Virus-like-particle; Vaccine; Baculovirus; Mouse model
期刊名称：VACCINE （ 影响因子：3.5； 五年影响因子：3.5 ）
ISSN： 0264-410X
年卷期： 2024 年 42 卷 25 期
页码：
收录情况： SCI
摘要： Getah virus (GETV) is an emerging mosquito-borne virus with economic impact on the livestock industry in East Asia. In this study, we successfully produced GETV virus-like particles (VLPs) in insect cells using the baculovirus expression vector system. We show that the GETV envelope glycoproteins were successfully expressed at the surface of the insect cell and were glycosylated. VLPs were isolated from the culture fluid as enveloped particles of 60-80 nm in diameter. Two 1 mu g vaccinations with this GETV VLP vaccine, without adjuvant, generated neutralizing antibody responses and protected wild-type C57/BL6 mice against GETV viremia and arthritic disease. The GETV VLP vaccine may find application as a horse and/or pig vaccine in the future.
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