Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus
文献类型： 外文期刊
作者： Wang, Yimin 1 ; Wang, Yong 2 ; Bi, Zhuangli 1 ; Wang, Jinbin 2 ; Wang, Gang 2 ; Ru, Xin 2 ; Meng, Chunchun 1 ; Zhu, Jie 1 ; Liu, Guangqing 1 ; Li, Chuanfeng 1 ;
作者机构： 1.Chinese Acad Agr Sci CAAS, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China
2.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Peoples R China
关键词： novel goose parvovirus; novel duck reovirus; simultaneous detection; one-step qPCR
期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN：
年卷期： 2025 年 13 卷 7 期
页码：
收录情况： SCI
摘要： The novel goose parvovirus (NGPV) and the novel duck reovirus (NDRV) are pathogens that can substantially affect the growth and development of ducklings, causing considerable economic losses to duck farms. Therefore, a timely, rapid, accurate, and high-throughput diagnosis and identification of viral infections are critical for preventing the spread of epidemics. In this study, a TaqMan probe-based duplex one-step RT-qPCR was established for the simultaneous detection and qualitative and quantitative identification of the two viruses. It demonstrated greater sensitivity than conventional PCR, detecting as low as 2.42 copies/mu L of NGPV genome and 70.1 copies/mu L of NDRV genome. Additionally, it exhibited remarkable specificity, responding exclusively to the nucleic acids of target pathogens. It also demonstrated excellent reproducibility and availability, particularly in clinical settings, with a coinfection detection rate of 13.3%, contributing to the development of NGPV- and NDRV-testing technologies.
- 相关文献
作者其他论文 更多>>
-
Structural insights into the mechanism of phosphate recognition and transport by XPR1
作者：Zhang, Wenhui;Chen, Yanke;Guan, Zeyuan;Tang, Meng;Du, Zhangmeng;Zhang, Jie;Cheng, Meng;Zuo, Jiaqi;Liu, Yan;Wang, Qiang;Liu, Yanjun;Zhang, Delin;Yin, Ping;Ma, Ling;Liu, Zhu;Wang, Yong;Liu, Zhu
关键词：
-
Identification of Novel 58-5p and SREBF1 Interaction and Effects on Apoptosis of Ovine Ovarian Granulosa Cell
作者：Yang, Ruochen;Wang, Yong;Yue, Sicong;Liu, Yueqin;Zhang, Yingjie;Duan, Chunhui;Yang, Ruochen;Wang, Yong
关键词：ovine ovarian GCs; PRL; apoptosis; novel 58-5p; SREBF1
-
Rapid introgression of the clubroot resistance gene CRa into cabbage skeleton inbred lines through marker assisted selection
作者：Zhang, Na;Zhu, Mingzhao;Qiu, Yuting;Fang, Zhiyuan;Zhuang, Mu;Zhang, Yangyong;Lv, Honghao;Ji, Jialei;Yang, Limei;Wang, Yong;Zhu, Mingzhao;Hou, Xilin
关键词：Clubroot; Breeding; Backcross; Marker assisted selection
-
From hemorrhage to apoptosis: understanding the devastating impact of ASFV on piglets
作者：Xu, Fanghua;Li, Ning;Xue, Yue;Wang, Zhenru;Fang, Zheng;An, Hui;Liu, Sidang;Wang, Gang;Weng, Changjiang;Huang, Li
关键词：ASFV; multiple organ lesions; pathogenicity; apoptosis; piglets
-
Metarhizium spp. encode an ochratoxin cluster and a high efficiency ochratoxin-degrading amidohydrolase revealed by genomic analysis
作者：Wang, Gang;Ma, Junning;Xing, Fuguo;Wu, Wenqing;Guo, Xu;Li, Erfeng;Keller, Nancy P.
关键词：Secondary metabolite; Mycotoxin; OTA; Detoxification; Ochratoxigenic fungi
-
Enhancing drying efficiency and physicochemical & antioxidant properties of germinated brown rice through microwave incorporation
作者：Tie, Xiaoyu;Huo, Da;Wan, Lina;Zhao, Dan;An, Nannan;Li, Dong;Wang, Lijun;Wang, Yong
关键词：Drying methods; Drying characteristics; Starch gelatinization; Antioxidant activity
-
Variation in a single allele drives divergent yield responses to elevated CO2 between rice subspecies
作者：Liu, Yunlong;Zhang, Siyu;Shen, Chengbo;Ding, Yanfeng;Li, Shan;Jiang, Yu;Liu, Yunlong;Zhang, Siyu;Qian, Haoyu;Shen, Chengbo;Wang, Songhan;Liu, Zhenghui;Li, Ganghua;Ding, Yanfeng;Li, Shan;Jiang, Yu;Liu, Yunlong;Qian, Haoyu;Wang, Songhan;Liu, Zhenghui;Li, Ganghua;Ding, Yanfeng;Jiang, Yu;Hu, Shuijin;Zhang, Weijian;Wang, Yong;Huang, Shan;Wang, Yong;Huang, Shan;Fu, Xiangdong;Fu, Xiangdong;van Groenigen, Kees Jan
关键词：
