Rapid introgression of the clubroot resistance gene CRa into cabbage skeleton inbred lines through marker assisted selection
文献类型： 外文期刊
作者： Zhang, Na 1 ; Zhu, Mingzhao 1 ; Qiu, Yuting 1 ; Fang, Zhiyuan 1 ; Zhuang, Mu 1 ; Zhang, Yangyong 1 ; Lv, Honghao 1 ; Ji, Jialei 1 ; Hou, Xilin 3 ; Yang, Limei 1 ; Wang, Yong 1 ;
作者机构： 1.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr,Key Lab Biol & Genet Imp, State Key Lab Vegetable Biobreeding,Beijing Key La, Beijing 100097, Peoples R China
3.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
关键词： Clubroot; Breeding; Backcross; Marker assisted selection
期刊名称：MOLECULAR BREEDING （ 影响因子：3.0； 五年影响因子：3.0 ）
ISSN： 1380-3743
年卷期： 2025 年 45 卷 2 期
页码：
收录情况： SCI
摘要： Clubroot, caused by Plasmodiophora brassicae, is a globally pervasive soil-borne disease that poses a significant challenge primarily in cruciferous crops. However, the scarcity of resistant materials and the intricate genetic mechanisms within cabbage present major obstacles to clubroot resistance (CR) breeding. In our previous research, we developed an Ogura CMS cabbage variety, "17CR3", which harbors the CRa gene, crucial for CR. The fertility of this variety can be restored through crossing with an Ogura cytoplasmic male sterile (CMS) restore line. In the current investigation, offspring from fertile hybrids were utilized as donor parents in backcrossing with five cabbage inbred lines, with the goal of introducing the CRa gene into elite cabbage cultivars possessing superior agronomic traits. Following five years of continuous field selection combined with molecular marker-assisted selection (MAS), we successfully developed BC4 individuals exhibiting excellent agronomic traits and diverse genetic backgrounds. Whole-genome resequencing revealed a mere 54,213 SNP differences between the genetic makeup of BC4 individuals and their recurrent parents. The results of inoculation identification demonstrated a high degree of co-segregation between the CRa-specific marker KBrH129J18 and resistance to Plasmodiophora brassicae in both inoculated resistant seedlings and cabbage plants harboring CRa across three distinct regions of China. Additionally, results from Semi-Quantitative RT-PCR experiments revealed minimal to no expression of CRa in the majority of susceptible individuals, underscoring the pivotal role of CRa in conferring CR. Moreover, BC3 individuals resulting from the cross between "SK308" and "18CR3", which carried CRa, exhibited resistance to clubroot under the natural conditions of disease-prone fields in Wulong, China. In summary, through a combination of traditional breeding methods and MAS, we successfully bred five cabbage inbred lines carrying the CRa gene from diverse genetic backgrounds, thereby establishing a robust foundation for their integration into breeding programs.
- 相关文献
作者其他论文 更多>>
-
Structural insights into the mechanism of phosphate recognition and transport by XPR1
作者：Zhang, Wenhui;Chen, Yanke;Guan, Zeyuan;Tang, Meng;Du, Zhangmeng;Zhang, Jie;Cheng, Meng;Zuo, Jiaqi;Liu, Yan;Wang, Qiang;Liu, Yanjun;Zhang, Delin;Yin, Ping;Ma, Ling;Liu, Zhu;Wang, Yong;Liu, Zhu
关键词：
-
Identification of Novel 58-5p and SREBF1 Interaction and Effects on Apoptosis of Ovine Ovarian Granulosa Cell
作者：Yang, Ruochen;Wang, Yong;Yue, Sicong;Liu, Yueqin;Zhang, Yingjie;Duan, Chunhui;Yang, Ruochen;Wang, Yong
关键词：ovine ovarian GCs; PRL; apoptosis; novel 58-5p; SREBF1
-
Enhancing drying efficiency and physicochemical & antioxidant properties of germinated brown rice through microwave incorporation
作者：Tie, Xiaoyu;Huo, Da;Wan, Lina;Zhao, Dan;An, Nannan;Li, Dong;Wang, Lijun;Wang, Yong
关键词：Drying methods; Drying characteristics; Starch gelatinization; Antioxidant activity
-
Estimating Stratified Biomass in Cotton Fields Using UAV Multispectral Remote Sensing and Machine Learning
作者：Hu, Zhengdong;Fan, Shiyu;Guo, Rensong;Wang, Liang;Zhang, Na;Cui, Jianping;Lin, Tao;Hu, Zhengdong;Fan, Shiyu;Tang, Qiuxiang;Bao, Longlong;Sarsen, Guldana;Li, Yabin;Zhang, Shuyuan;Jin, Xiuliang
关键词：UAV multispectral sensing; aboveground biomass; stratified estimation; vegetation index; machine learning; precision agriculture
-
EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang
关键词：metagenome; microbiome; microbiota; pipeline; visualization
-
Variation in a single allele drives divergent yield responses to elevated CO2 between rice subspecies
作者：Liu, Yunlong;Zhang, Siyu;Shen, Chengbo;Ding, Yanfeng;Li, Shan;Jiang, Yu;Liu, Yunlong;Zhang, Siyu;Qian, Haoyu;Shen, Chengbo;Wang, Songhan;Liu, Zhenghui;Li, Ganghua;Ding, Yanfeng;Li, Shan;Jiang, Yu;Liu, Yunlong;Qian, Haoyu;Wang, Songhan;Liu, Zhenghui;Li, Ganghua;Ding, Yanfeng;Jiang, Yu;Hu, Shuijin;Zhang, Weijian;Wang, Yong;Huang, Shan;Wang, Yong;Huang, Shan;Fu, Xiangdong;Fu, Xiangdong;van Groenigen, Kees Jan
关键词：
-
Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus
作者：Wang, Yimin;Bi, Zhuangli;Meng, Chunchun;Zhu, Jie;Liu, Guangqing;Li, Chuanfeng;Wang, Yimin;Wang, Yong;Wang, Jinbin;Wang, Gang;Ru, Xin
关键词：novel goose parvovirus; novel duck reovirus; simultaneous detection; one-step qPCR
