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Characterization of azadirachtin resistance in a laboratory-selected strain of Drosophila. melanogaster

文献类型： 外文期刊

作者： Liu, Kuitun 1 ; Rao, Cong 1 ; Li, Chongwei 1 ; Jiang, Wenlin 1 ; Hu, Bo 2 ; Su, Jianya 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, State Key Lab Agr & Forestry Biosecur, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural & Anim Biotechnol, Zhenjiang 212100, Peoples R China

3.Chinese Acad Agr Sci, Sericultural Sci Res Ctr, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang 212100, Peoples R China

关键词： Azadirachtin; Resistance inheritance; Cross-resistance; Fitness costs; Metabolic resistance

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2025 年 212 卷

页码：

收录情况： SCI

摘要： Azadirachtin, a natural sesquiterpenoid compound, exhibits broad-spectrum insecticidal activity, yet its mechanism of action remains incompletely understood. This study aimed to elucidate its toxicological mechanisms by developing an azadirachtin-resistant Drosophila strain through continuous laboratory selection. After five years of directional screening, we successfully established the Aza-Sel strain with a resistance ratio of 86.7-fold. Genetic analysis revealed that the resistance trait is incompletely recessive and polygenic in nature. Detoxification enzyme and synergism assays demonstrated that elevated activities of glutathione-S-transferases and esterases play crucial roles in the metabolic resistance of this strain. Cross-resistance spectrum analysis showed that the Aza-Sel strain exhibited 12.4-fold and 6.7-fold cross-resistance to emamectin benzoate and dinotefuran, respectively, while displaying negative cross-resistance to chlorpyrifos and indoxacarb, with no significant crossresistance to other tested insecticides. Notably, the resistant strain exhibited substantial fitness costs: significant developmental delays across all stages (egg, larva, pupa), prolonged developmental duration, markedly reduced fecundity and egg hatch rate, and significantly shortened male longevity. These findings indicate that the azadirachtin-resistant strain bears severe fitness costs, which may account for the slow development of resistance under laboratory selection conditions. The results confirm the significant advantages of azadirachtin in integrated pest management, particularly its negative cross-resistance characteristics with emamectin benzoate and indoxacarb, making it an ideal option for managing resistant pests.

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