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DGAT1-mediated lipid metabolism is essential for female reproduction in the silkworm, Bombyx mori

文献类型： 外文期刊

作者： Xing, Zhiping 1 ; Lu, Tao 1 ; Deng, Yuping 1 ; Zhang, Yuting 1 ; Dong, Hui 1 ; Li, Zhipeng 1 ; Hu, Bo 1 ; Tan, Anjiang 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural & Anim Biotechnol, Zhenjiang 212100, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Sci Res Ctr, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang, Peoples R China

关键词： Bombyx mori; DGAT1; female reproduction; CRISPR/Cas9; lipid metabolism

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）

ISSN： 1526-498X

年卷期： 2025 年

页码：

收录情况： SCI

摘要： BACKGROUNDLipid metabolism plays a critical role in maintaining energy homeostasis during various physiological processes in insects. Diacylglycerol O-acyltransferase-1 (DGAT1) is associated with the synthesis of triacylglycerol (TAG), a process that is necessary for the absorption of dietary fats and the storage of fat in mammals. However, the biological roles of DGAT1 in insects remain largely unknown.RESULTSIn this study, we identified and characterized the DGAT1 gene in Bombyx mori. Temporal and spatial expression profiles showed that BmDGAT1 was broadly expressed in all examined tissues and had higher expression in the midgut and gonads. BmDGAT1 mutants were obtained by a binary transgenic CRISPR/Cas9. Disruption of BmDGAT1 resulted in a significant decrease in the body weight of larvae and pupae, and reduced the content of TAG. In addition, deletion of BmDGAT1 hindered ovarian development and completely inhibited the formation of mature eggs in female adults. Meanwhile, a dramatic reduction in the TAG content was observed in the ovaries of BmDGAT1 mutants. Furthermore, the RNA-sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) analyses revealed that knockout of BmDGAT1 affected the expression of genes associated with female reproduction in the mutants.CONCLUSIONAltogether, DGAT1-mediated lipid metabolism plays a key role in regulating silkworm female reproduction, which is a potential target for controlling lepidopteran pests. (c) 2025 Society of Chemical Industry.

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