The Isolation and Construction of an Infectious Clone for a Duck Adenovirus Type 3 Strain
文献类型： 外文期刊
作者： Lu, Haipeng 1 ; Tang, Mei 2 ; Zhang, Zhifei 2 ; Wu, Mi 1 ; Yuan, Chunxiu 2 ; Pan, Xue 2 ; Liu, Qinfang 2 ; Teng, Qiaoyang 2 ; Xu, Bangfeng 2 ; Yan, Minghao 2 ; Yan, Dawei 2 ; Wang, Fenglong 1 ; Li, Zejun 1 ;
作者机构： 1.Inner Mongolia Agr Univ, Coll Vet Med, Hohhot 010010, Peoples R China
2.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Dept Avian Infect Dis, Shanghai 200241, Peoples R China
关键词： duck adenovirus type 3 (DAdV-3); infectious clone; Hexon gene; biological characteristics
期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN：
年卷期： 2025 年 13 卷 6 期
页码：
收录情况： SCI
摘要： A duck adenovirus type 3 strain, SD2019, was isolated from sick Muscovy ducks in our laboratory in 2019. To study the biological properties of the virus, an infectious clone of the SD2019 strain was successfully established. The plasmid containing the whole genome of DAdV-3 was digested with Pac I and the linearized DNAs were electortransfected into LMH cells; the cells showed cytopathic effects (CPEs) at 96 h post transfection and the rescued virus (rSD2019) was identified by PCR and indirect immunofluorescence assays (IFAs). The biological characteristics of strain rSD2019 were studied in vitro and in vivo and the results show that rSD2019 grew to similar titers as compared with the wild-type SD2019 strain in LMH cells, as well as showing similar replication and virulence in Muscovy ducks. The establishment of a reliable reverse genetics system for DAdV-3 provides a foundation for future studies of DAdV-3.
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