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Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome

文献类型： 外文期刊

作者： Zhang, Zhifei 1 ; Su, Xin 1 ; Shuo, Dun 1 ; Yan, Dawei 1 ; Pan, Xue 1 ; Xu, Bangfeng 1 ; Yan, Minghao 1 ; Ren, Shuxuan 1 ; Liu, Qinfang 1 ; Yuan, Chunxiu 1 ; Teng, Qiaoyang 1 ; Li, Zejun 1 ;

作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Dept Avian Infect Dis, Shanghai 200241, Peoples R China

关键词： DERSV; RT-qPCR; TaqMan; specificity; sensitivity

期刊名称：VETERINARY SCIENCES （ 影响因子：2.3； 五年影响因子：2.4 ）

ISSN：

年卷期： 2025 年 12 卷 3 期

页码：

收录情况： SCI

摘要： Duck egg-reducing syndrome virus (DERSV) is a novel Avihepatovirus and is responsible for a gradual decline in the laying rate of ducks, decreasing from a peak of 90% to 50%. The development of a rapid detection method for DERSV is crucial for the identification and control of virus infections. In this study, we developed a quantitative reverse transcription PCR (RT-qPCR) assay for detecting DERSV. Specific primers and a probe were designed to target a conserved region of the 3D gene. The assay demonstrated high specificity, with no cross-reactivity to other non-target duck viruses. It had a detection limit of 102 copies and a linear range from 102 to 109 copies per reaction. The assay's efficiency was 92.59%, with a regression coefficient (R2) of 0.999. The coefficient of variation for both intra-and inter-assays was less than 2.00%. Among the 153 clinical samples collected from 2016 to 2023, the RT-qPCR detected a DERSV positive ratio of 47.06% (72/153). In conclusion, the utilization of the real-time RT-qPCR assay holds potential for the detection of DERSV in epidemiological and pathogenesis studies.

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