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A simple and low-cost method for fluoride analysis of plant materials using alkali extraction and ion-selective electrode

文献类型： 外文期刊

作者： Zhang, Chenyu 1 ; Aarts, Mark G. M. 1 ; van der Ent, Antony 1 ;

作者机构： 1.Wageningen Univ & Res, Lab Genet, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands

2.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Biol Genet & Breeding Special Econ Anim &, Tea Res Inst, Hangzhou 310008, Peoples R China

关键词： Chemometrics; Orthogonal assay design; Fluoride; Plant research; Ion-selective electrode

期刊名称：PLANT METHODS （ 影响因子：4.4； 五年影响因子：5.7 ）

ISSN：

年卷期： 2025 年 21 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundsExisting methods for fluoride (F-) determination in plant material require expensive equipment and specialized reagents. This study aimed to develop a simple and cost-effective method for fluoride analysis in plant samples.ResultsUsing an orthogonal assay design with certified reference material, this study optimized a sodium hydroxide extraction method (5 molL-1) with heating at 120 degrees C for 0.5 h, followed by the addition of potassium acetate, ionic strength adjustment, and measurement via an ion-selective electrode. The method achieved a limit of detection (LOD) and limit of quantification (LOQ) of 1.41 and 4.71 mgkg(-)(1), respectively. Recovery rates ranged from 84.74 to 89.34% in Arabidopsis thaliana (intraday relative standard deviation [RSD] <= 2.31%, inter-day RSD <= 4.17%) and from 83.53 to 91.55% in Camellia sinensis (intraday RSD <= 3.11%, inter-day RSD <= 4.98%). In A. thaliana cultivated in NaF-dosed (500 mu M) nutrient solution, the fluoride concentration in the shoot was 16.00 mgkg-1; In C. sinensis grown under 250 mu M NaF treatment, the shoot fluoride concentration was 292.71 mgkg-1. Moreover, the fluoride concentration in Tea products purchased from local supermarkets ranged from 16.28 to 61.78 mg kg-1.ConclusionThis study presents a simple, reliable, and cost-effective method for fluoride analysis in plant materials, which can be further validated through inter-laboratory testing to establish a standardized approach.

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