CsEXL3 regulate mechanical harvest-related droopy leaves under the transcriptional activation of CsBES1.2 in tea plant
文献类型： 外文期刊
作者： Liu, Haoran 1 ; Duan, Lingxiao 1 ; Ma, Jianqiang 1 ; Jin, Jiqiang 1 ; Huang, Rong 1 ; Liu, Yujie 1 ; Chen, Si 1 ; Xu, Xiaoying 1 ; Chen, Jiedan 1 ; Yao, Mingzhe 1 ; Chen, Liang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Biol Genet & Breeding Special Econ Anim &, Tea Res Inst, Hangzhou 310008, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.7； 五年影响因子：9.0 ）
ISSN： 2662-6810
年卷期： 2024 年 11 卷 5 期
页码：
收录情况： SCI
摘要： Due to a labor shortage, the mechanical harvesting of tea plantations has become a focal point. However, mechanical harvest efficiency was hampered by droopy leaves, leading to a high rate of broken tea shoots and leaves. Here, we dissected the genetic structure of leaf droopiness in tea plants using genome-wide association studies (GWAS) on 146 accessions, combined with transcriptome from two accessions with contrasting droopy leaf phenotypes. A set of 16 quantitative trait loci (QTLs) containing 54 SNPs and 34 corresponding candidate genes associated with droopiness were then identified. Among these, CsEXL3 (EXORDIUM-LIKE 3) from Chromosome 1 emerged as a candidate gene. Further investigations revealed that silencing CsEXL3 in tea plants resulted in weaker vascular cell malformation and brassinosteroid-induced leaf droopiness. Additionally, brassinosteroid signal factor CsBES1.2 was proved to participate in CsEXL3-induced droopiness and vascular cell malformation via using the CsBES1.2-silencing tea plant. Notably, CsBES1.2 bound on the E-box of CsEXL3 promoter to transcriptionally activate CsEXL3 expression as CUT&TAG based ChIP-qPCR and ChIP-seq suggested in vivo as well as EMSA and Y1H indicated in vitro. Furthermore, CsEXL3 instead of CsBES1.2 decreased lignin content and the expressing levels of lignin biosynthesis genes. Overall, our findings suggest that CsEXL3 regulates droopy leaves, partially through the transcriptional activation of CsBES1.2, with the potential to improve mechanical harvest efficiency in tea plantations.
- 相关文献
作者其他论文 更多>>
-
Responsive root traits and mitigating strategies for wheat production under single or combined abiotic stress
作者：Chen, Si;Long, Lizhi;Sun, Xiaolei;Parsons, David;Zhou, Zhenjiang
关键词：Root traits; Multiple abiotic stress; Root plasticity; Wheat production; Agronomic practices
-
Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus
作者：Zhang, Shunfen;Zhong, Ruqing;Li, Kai;Wang, Huixin;Xu, Ye;Liu, Dadan;Chen, Liang;Zhang, Hongfu;Zhang, Shunfen;Lv, Huiyuan;Ma, Qiugang;Zhou, Miao
关键词：baicalin;
E. coli ; intestinal inflammation;Lactobacillus amylovorus ; macrophages polarization; TLR4 -
Two leucine-rich repeat receptor-like kinases initiate herbivory defense responses in tea plants
作者：Jiang, Qi;Ding, Changqing;Feng, Lingjia;Wu, Zhenwei;Liu, Yujie;He, Lintong;Liu, Chuande;Wang, Lu;Zeng, Jianming;Huang, Jianyan;Ye, Meng
关键词：
-
Chitooligosaccharide application enhanced the growth and phytoremediation efficiency of industrial hemp in Cd-contaminated soils
作者：Guo, Yuan;Zhao, Xinlin;Wang, Yuanchang;Wang, Chenyuan;Zhou, Wei;Huang, Rong;Guo, Yuan;Zhao, Xinlin;Wang, Yuanchang;Wang, Chenyuan;Zhou, Wei;Huang, Rong;Guo, Yuan;Zhao, Xinlin;Ouyang, Da;Gao, Shuaishuai;Tan, Xiaofei;Tan, Xiaofei;Tan, Xiaofei
关键词：Antioxidant; Bioconcentration; Biomass; Cannabis sativa; Heavy metal; Phytomanagement
-
Integration of digital phenotyping, GWAS, and transcriptomic analysis revealed a key gene for bud size in tea plant (Camellia sinensis)
作者：Zhang, Shuran;Chen, Si;Fu, Zhilu;Li, Fang;Chen, Qiyu;Ma, Jianqiang;Chen, Liang;Chen, Jiedan;Chen, Yuanquan
关键词：
-
A cellulose-degrading Bacillus altitudinis from Tibetan pigs improved the in vitro fermentation characteristics of wheat bran
作者：Wang, Junhong;Ma, Teng;Xie, Yining;Li, Kai;Luo, Chengzeng;Teng, Chunran;Yi, Bao;Chen, Liang;Zhang, Hongfu
关键词：Cellulose-degrading bacteria; Wheat bran; Tibetan pigs; Fermentation
-
Identify Tea Plantations Using Multidimensional Features Based on Multisource Remote Sensing Data: A Case Study of the Northwest Mountainous Area of Hubei Province
作者：Xiao, Pengnan;Qian, Jianping;Yu, Qiangyi;Lin, Xintao;Xu, Jie;Liu, Yujie
关键词：Google Earth Engine; tea plantation; Sentinel-1; Sentinel-2; feature selection
