Nucleophosmin 1 inhibits the replication of influenza A virus by competitively binding viral RNA with viral proteins
文献类型： 外文期刊
作者： Yu, Yingying 1 ; Wang, Qian 1 ; Wei, Yanli 1 ; Liu, Junwen 1 ; Wang, Guangwen 2 ; Wang, Zhengxiang 1 ; Shen, Wentao 1 ; Han, Lu 1 ; Li, Chengjun 2 ; Lei, Cao-Qi 3 ; Xu, Shuai 1 ; Zhu, Qiyun 1 ;
作者机构： 1.Chinese Acad Agr Sci, Lanzhou Univ, Lanzhou Vet Res Inst, Coll Vet Med,State Key Lab Anim Dis Control & Prev, Lanzhou 730000, Peoples R China
2.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Harbin 150069, Peoples R China
3.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China
4.Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Lanzhou 730046, Peoples R China
关键词： RNA binding; Viral RNA; Viral polymerase; Nucleophosmin-1 (NPM1); Influenza A virus (IAV)
期刊名称：VIROLOGICA SINICA （ 影响因子：4.0； 五年影响因子：4.1 ）
ISSN： 1674-0769
年卷期： 2025 年 40 卷 3 期
页码：
收录情况： SCI
摘要： Influenza A viruses (IAVs) are single-stranded negative-sense RNA viruses that continually challenge animal and human health. In IAV-infected cells, host RNA-binding proteins play key roles in the life cycle of IAV by directly binding to viral RNA. Here, we examined the role of the host RNA-binding protein nucleophosmin-1 (NPM1) in IAV replication. We found that, as a nucleolar phosphoprotein, NPM1 directly binds to viral RNA (vRNA) and inhibits the replication of various subtypes of IAV. NPM1 binding to vRNA competitively reduces the assembly of the viral ribonucleoprotein complex and the viral polymerase activity, thereby reducing the generation of progeny viral RNA and virions. The RNA-binding activity of NPM1, with the key residues T199, T219, T234, and T237, is essential for its anti-influenza function. Taken together, our findings demonstrate that NPM1 acts as an RNA-binding protein and interacts with IAV vRNA to suppress viral replication.
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