The knockout of tobacco NtCBL10 inhibits leaf Cl- accumulation and leads to light-dependent necrosis & light-independent chlorosis under salt stress
文献类型： 外文期刊
作者： Mao, Jingjing 1 ; Yuan, Guang 1 ; Visser, Richard G. F. 3 ; Bai, Yuling 3 ; Xu, Gang 6 ; Xue, Lin 6 ; Mao, Dongping 6 ; Liu, Haobao 1 ; Ning, Yang 1 ; Wang, Qian 1 ; van der Linden, C. Gerard 3 ;
作者机构： 1.Chinese Acad Agr Sci CAAS, Tobacco Res Inst, Qingdao 266101, Peoples R China
2.Grad Sch Chinese Acad Agr Sci GSCAAS, Beijing 100081, Peoples R China
3.Wageningen Univ & Res WUR, Plant Breeding, NL-6708 PB Wageningen, Netherlands
4.Wageningen Univ, Grad Sch Expt Plant Sci, NL-6708 PB Wageningen, Netherlands
5.China Tobacco Jiangsu Ind Co Ltd, Technol Ctr, Nanjing 210000, Peoples R China
6.Anhui Wannan Tobacco Co Ltd, Xuancheng 242000, Peoples R China
关键词： CBL10; Salt tolerance; Necrosis; Chlorosis; Light
期刊名称：ENVIRONMENTAL AND EXPERIMENTAL BOTANY （ 影响因子：4.7； 五年影响因子：5.4 ）
ISSN： 0098-8472
年卷期： 2025 年 229 卷
页码：
收录情况： SCI
摘要： CBL10 was shown to be a key gene for salt tolerance in Arabidopsis thaliana. In this study, we evaluated the role of CBL10 in the tobacco salt tolerance response by characterizing the gene editing-induced loss-of-function knockout mutants of the two NtCBL10 homeologous genes NtCBL10A and NtCBL10B. The importance of NtCBL10 for the response to salinity was evidenced by the salt supersensitivity of the Nt-cbl10a10b double mutants, with fast-developing chlorosis and severe necrotic lesions on leaves. Stomatal conductance and photochemical efficiency of photosystem 2 (PhiPS2) of the Nt-cbl10a10b double mutant were significantly inhibited already at a very early stage of the salt stress response. Leaf Na+ concentrations were not much affected in these plants, but the Cl- content of the Nt-cbl10a10b double mutants was significantly lower than that of wild-type plants, which is the first report of CBL10 in the regulation of Cl- homeostasis. Interestingly, the necrosis phenotype of Ntcbl10a10b double mutants was dependent on light, while the chlorosis phenotype of Nt-cbl10a10b double mutants was light-independent. Different from the previous studies that focus on the role of CBL10 in Na+ homeostasis regulation, this study indicates that NtCBL10 is a key component in regulating multiple aspects of ion homeostasis under salt stress.
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