Isolation and evaluation of Bacillus subtilis RSS-1 as a potential biocontrol agent against Sclerotinia sclerotiorum on oilseed rape
文献类型： 外文期刊
作者： Cao, Shun 1 ; Jiang, Bingxin 1 ; Yang, Guogen 1 ; Pan, Guangxue 1 ; Pan, Yuemin 1 ; Chen, Fangxin 1 ; Gao, Zhimou 1 ; Dai, Yuli 2 ;
作者机构： 1.Anhui Agr Univ, Coll Plant Protect, Anhui Prov Key Lab Integrated Pest Management Crop, 130 West Changjiang Rd, Hefei 230036, Peoples R China
2.Fujian Acad Agr Sci, Inst Plant Protect, Fujian Key Lab Monitoring & Integrated Management, Fuzhou 350013, Peoples R China
关键词： Bacillus subtilis RSS-1; Identification; Sclerotinia sclerotiorum; Sclerotinia stem rot of oilseed rape; Biological control
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.8； 五年影响因子：2.0 ）
ISSN： 0929-1873
年卷期： 2023 年 166 卷 1 期
页码：
收录情况： SCI
摘要： Sclerotinia stem rot caused by Sclerotinia sclerotiorum is one of the most important diseases of oilseed rape in the world. Because of the absence of resistant varieties and the disadvantages of chemical control, application of antifungal microbes has become an eco-friendly and effective measure to control this disease. In this study, Bacillus subtilis strain RSS-1, isolated from soil samples, was identified based on morphological, physiological and biochemical tests, and DNA gyrase subunit A (gyrA), gyrB, DNA-directed RNA polymerase subunit beta (rpoB) and rpoC gene sequence analysis. It significantly inhibited mycelial growth and sclerotial production of S. sclerotiorum in vitro. In greenhouse experiments, all three tested concentrations (10(6), 10(7), 10(8) cfu mL(-1)) of cell fermentation broth and culture filtrate significantly reduced the severity of sclerotinia stem rot on oilseed rape (P < 0.05). RSS-1 was more effective at reducing disease severity when applied 24 h before inoculation with S. sclerotiorum than at 24 h post inoculation, suggesting that RSS-1 should be applied as a prophylactic rather than a curative biological agent. Colonization tests indicated that the population density of RSS-1 on rapeseed leaves significantly decreased (P < 0.05) over 6 days. However, RSS-1 could stably colonize in rhizospheric soil of rapeseed over 30 days. Challenge inoculation tests showed that RSS-1 significantly inhibited the activities of polygalacturonase and cellulase and accumulation of oxalic acid during the S. sclerotiorum infection. These results suggest that RSS-1 was a potential biological agent for controlling sclerotinia stem rot caused by S. sclerotiorum on oilseed rape.
- 相关文献
作者其他论文 更多>>
-
Occurrence of leaf spot caused by Colletotrichum plurivorum on cowpea in Fujian Province, China
作者：Dai, Yuli;Gan, Lin;Liu, Xiaofei;Lan, Chengzhong;Yang, Xiujuan;Li, Zhiqiang
关键词：Cowpea anthracnose; Colletotrichum plurivorum; Molecular identification; Pathogenicity; Vigna unguiculata
-
Population structure and mixed reproductive strategies in Bipolaris maydis from single and multiple corn cultivars in Fujian Province, China
作者：Dai, Yuli;Gan, Lin;Lan, Chengzhong;Liu, Xiaofei;Yang, Xiujuan;Liu, Wende
关键词：Bipolaris maydis; reproductive strategy; genetic structure; haplotypic diversity; genetic differentiation; mating type
-
Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Specific and Sensitive Detection of Mycocentrospora acerina (Hart.) Causing Round Leaf Spot Disease in Sanqi (Panax notoginseng)
作者：Lan, Chengzhong;Gan, Lin;Dai, Yuli;Liu, Xiaofei;Yang, Xiujuan
关键词：Panax notoginseng; molecular diagnosis; disease detection; Mycocentrospora acerina; loop-mediated isothermal amplification (LAMP)
-
Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China
作者：Dai, Yuli;Gan, Lin;Lan, Chengzhong;Lu, Xuesong;Yang, Xiujuan;Gao, Zhimou
关键词：Setosphaeria turcica; northern corn leaf blight; mating type; genetic diversity; population structure; sexual reproduction
-
Characterization of Natural Isolates of Bipolaris maydis Associated with Mating Types, Genetic Diversity, and Pathogenicity in Fujian Province, China
作者：Dai, Yuli;Gan, Lin;Ruan, Hongchun;Shi, Niuniu;Du, Yixin;Chen, Furu;Yang, Xiujuan
关键词：Bipolaris maydis; mating type; genetic diversity; pathogenicity; southern corn leaf blight
-
Characterization of Natural Isolates of Bipolaris maydis Associated with Mating Types, Genetic Diversity, and Pathogenicity in Fujian Province, China
作者：Dai, Yuli;Gan, Lin;Ruan, Hongchun;Shi, Niuniu;Du, Yixin;Chen, Furu;Yang, Xiujuan
关键词：Bipolaris maydis; mating type; genetic diversity; pathogenicity; southern corn leaf blight
-
Sensitivity of Cochliobolus heterostrophus to three demethylation inhibitor fungicides, propiconazole, diniconazole and prochloraz, and their efficacy against southern corn leaf blight in Fujian Province, China
作者：Dai, Yuli;Gan, Lin;Ruan, Hongchun;Shi, Niuniu;Du, Yixin;Wei, Zhixia;Chen, Furu;Yang, Xiujuan;Liao, Lei;Teng, Zhenyong
关键词：Demethylation inhibitor fungicide; Cochliobolus heterostrophus; Sensitivity; Cross-resistance; Control efficacy
