Simultaneous quantification of oxyclozanide and levamisole in milk via ultra-high-performance liquid chromatography tandem mass spectrometry: A validated high-sensitivity approach for veterinary residue surveillance
文献类型： 外文期刊
作者： Dai, G. 1 ; Sun, K. 1 ; Zhou, Y. 1 ; Li, B. 1 ; Wang, Q. 1 ; Qiu, Y. 1 ; Peng, X. 3 ; Zhou, X. 1 ; Zhang, J. 1 ;
作者机构： 1.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Vet Pharmaceut Dev, Minist Agr & Rural Affairs, Lanzhou 730050, Peoples R China
2.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China
3.Lanzhou Biopharmaceut Factory China Anim Husb Ind, Lanzhou 730102, Peoples R China
关键词： veterinary drug residues; UPLC-MS/MS; food safety; antiparasitic agents; milk
期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）
ISSN： 0022-0302
年卷期： 2025 年 108 卷 8 期
页码：
收录情况： SCI
摘要： This study developed a novel analytical method integrating liquid-liquid extraction with solid-phase extraction for simultaneous detection of oxyclozanide (OXY) and levamisole hydrochloride (LMS) residues in milk using ultra-high-performance liquid chromatography tandem MS (UPLC-MS/MS). Method validation demonstrated exceptional sensitivity with detection limits of 1.00 ng/mL and quantitation limits of 3.00 ng/mL for both analytes. Low matrix effects (CV <8.00%) and satisfactory recoveries (OXY: 84.00%-108.77%, relative SD [RSD]: 2.20%-8.00%; LMS: 89.50%-119.60%, RSD: 2.10%-8.36%) were achieved across multiple validation parameters, while sample stability remained consistent under varied processing conditions (CV <10.00%). Analysis of 120 commercial milk samples revealed no detectable residues, confirming both the reliability of this monitoring framework and the compliance of Chinese dairy products with international safety standards. As the first reported method enabling concurrent detection of this antiparasitic drug combination, the established protocol provides crucial technical support for veterinary drug residue surveillance, ensuring safer application of compound oxyclozanide suspension in livestock husbandry.
- 相关文献
作者其他论文 更多>>
-
Development and validation of a liquid chromatography-mass spectrometry method for free monosaccharide analysis in milk from 8 species
作者：Qi, Y. J.;Lu, S. C.;Zhao, X. W.;Chen, M. Q.;Song, L.;Zhang, J.;Zheng, N.;Wang, J. Q.;Zhang, Y. D.;Qi, Y. J.;Lu, S. C.;Zhao, X. W.;Chen, M. Q.;Song, L.;Zhang, J.;Zheng, N.;Wang, J. Q.;Zhang, Y. D.;Qi, Y. J.;Lu, S. C.;Zhao, X. W.;Chen, M. Q.;Song, L.;Zhang, J.;Zheng, N.;Wang, J. Q.;Zhang, Y. D.
关键词：free monosaccharide; milk composition; LC-MS; method validation; neonatal nutrition
-
The Other Side of Defensins: Review of Antiviral Activity of Defensins (Review)
作者：Tao, K.;He, C.;Zhu, Y.;Li, Z.;Shao, D.;Li, B.;Wei, J.;Qiu, Y.;Ma, Z.;Liu, K.
关键词：
-
Effects of energy restriction during growing phase on the productive performance of Hyline Brown laying hens aged 6 to 72 wk
作者：Lu, J.;Wang, Q.;Wang, K. H.;Ma, M.;Wang, X. G.;Guo, J.;Dou, T. C.;Hu, Y. P.;Li, Y. F.;Qu, L.;Lu, J.;Yang, Z.
关键词：laying hen; energy consumption during growing phase; productive performance; sexual organ development; sexual maturity
-
Expression and self-assembly of virus-like particles from porcine parvovirus and its application in antibody detection
作者：Li, Y.;Yue, W.;Li, X.;Chen, Y.;Gao, Y.;Li, Y.;Yue, W.;Li, X.;Chen, Y.;Gao, Y.;Wang, Q.
关键词：porcine parvovirus; virus -like particles; indirect ELISA
-
Using combined Lactobacillus and quorum quenching enzyme supplementation as an antibiotic alternative to improve broiler growth performance, antioxidative status, immune response, and gut microbiota
作者：Sun, X. X.;Chen, D.;Deng, S. Q.;Sa, R. N.;Zhang, G. M.;Peng, X.
关键词：broiler; Lactobacillus; quorum quenching enzyme; growth performance; microbiota
-
Annual analysis of field-evolved insecticide resistance in Bemisia tabaci across China
作者：Zheng, H.;Xie, W.;Fu, B.;Xiao, S.;Tan, X.;Ji, Y.;Cheng, J.;Yang, X.;Guo, Z.;Wang, S.;Wu, Q.;Xu, B.;Zhang, Y.;Zheng, H.;Fu, B.;Wang, R.;Liu, B.;Zhou, X.
关键词：Bemisia tabaci; resistance; insecticides; spirotetramat; cyantraniliprole
-
Dynamic infection ofVerticillium dahliaein upland cotton
作者：Zhang, J.;Hu, H. -L.;Wang, X. -N.;Yang, Y. -H.;Tang, C. -M.;Zhang, C. -J.;Zhu, H. -Q.;Shi, L.;Zhao, M. -W.
关键词：immunity response; infection mechanism; pathogenicity; resistance; upland cotton; Verticillium dahliae
