文献类型： 外文期刊
作者： Liu, Xin 1 ; Wu, Jun 1 ; Peng, Yixiu 1 ; Qian, Hongwu 1 ; Lv, Xiaoqian 1 ; Li, Fan 1 ; Jin, Kai 1 ; Niu, Yingjie 1 ; Song, Jiuzhou 3 ; Han, Wei 4 ; Chen, Guohong 1 ; Li, Bichun 1 ; Zuo, Qisheng 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Mol Design Jiangsu P, Yangzhou 225009, Peoples R China
2.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou 225009, Peoples R China
3.Univ Maryland, Anim & Avian Sci, College Pk, MD 20742 USA
4.Chinese Acad Agr Sci Poultry Inst Jiangsu, Poultry Inst, Yangzhou 225003, Peoples R China
5.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212100, Peoples R China
关键词： primordial germ cells; insulin; RNA-seq; proliferation
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 7 期
页码：
收录情况： SCI
摘要： Insulin is an important component of stem cell cultures; however, its role in the proliferation of avian primordial germ cells (PGCs) is unknown. The proliferation of PGCs in cultures varies and the growth factors and signaling pathways necessary to induce the proliferation of PGCs in chickens are unknown. Therefore, we conducted the present study to investigate the effect of insulin on the survival and proliferation of PGCs. In this study, we observed that under this culture system, PGCs proliferate in the presence of insulin, but do not proliferate in the absence of insulin. Furthermore, in insulin-lacking media, the expression of pluripotency genes, including LIN28, NANOG, POUV, and SOX2, was markedly decreased. Similarly, the expression of cell adhesion proteins ZO-1, Occludin, and JAM-A was significantly reduced. Elevated levels of ROS, GSSG, and MDA reduced the redox capacity of the cells and induced apoptosis. Subsequent transcriptome analyses revealed that insulin is one of the key factors in the proliferation of chicken PGCs through the regulation of downstream genes by PI3K/AKT, ECM-receptor interaction, Wnt, and P53 signaling, and that these downstream genes may be important for PGCs' proliferation and survival.
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