Heat-labile enterotoxin enhances F4-producing enterotoxigenic E. coli adhesion to porcine intestinal epithelial cells by upregulating bacterial adhesins and STb enterotoxin
文献类型: 外文期刊
作者: Duan, Qiangde 1 ; Pang, Shengmei 1 ; Feng, Lili 4 ; Liu, Jiaqi 1 ; Lv, Linfen 1 ; Li, Baoliang 1 ; Liang, Yuxuan 1 ; Zhu, Guoqiang 1 ;
作者机构: 1.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China
2.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
3.Jiangsu Joint Lab Int Cooperat Prevent & Control, Yangzhou 225009, Jiangsu, Peoples R China
4.Henan Acad Agr Sci, Inst Agr Econ & Informat, Zhengzhou 450002, Peoples R China
关键词: Heat-labile enterotoxin; ETEC; adherence; fimbriae; pathogenesis
期刊名称:VETERINARY RESEARCH ( 影响因子:3.829; 五年影响因子:4.269 )
ISSN: 0928-4249
年卷期: 2022 年 53 卷 1 期
页码:
收录情况: SCI
摘要: As one of the crucial enterotoxins secreted by enterotoxigenic Escherichia coli (ETEC), heat-labile enterotoxin (LT) enhances bacterial adherence both in vivo and in vitro; however, the underlying mechanism remains unclear. To address this, we evaluated the adherence of LT-producing and LT-deficient ETEC strains using the IPEC-J2 cell model. The expression levels of inflammatory cytokines and chemokines, and tight-junction proteins were evaluated in IPEC-J2 cells after infection with various ETEC strains. Further, the levels of adhesins and enterotoxins were also evaluated in F4ac-producing ETEC (F4 + ETEC) strains after treatment with cyclic AMP (cAMP). The adherence of the Delta eltAB mutant was decreased compared with the wild-type strain, whereas adherence of the 1836-2/pBR322-eltAB strain was markedly increased compared with the 1836-2 parental strain. Production of LT up-regulated the expression of TNF-alpha, IL-6, CXCL-8, and IL-10 genes. However, it did not appear to affect tight junction protein expression. Importantly, we found that cAMP leads to the upregulation of adhesin production and STb enterotoxin. Moreover, the F4 + ETEC strains treated with cAMP also had greater adhesion to IPEC-J2 cells, and the adherence of Delta faeG, Delta fliC, and Delta estB mutants was decreased. These results indicate that LT enhances the adherence of F4 + ETEC due primarily to the upregulation of F4 fimbriae, flagellin, and STb enterotoxin expression and provide insights into the pathogenic mechanism of LT and ETEC.
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