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Unrevealing the therapeutic potential of artesunate against emerging zoonotic Babesia microti infection in the murine model

文献类型： 外文期刊

作者： Fazilani, Saqib Ali 1 ; An, Wei 3 ; Li, Sihong 4 ; Hassan, Mohammad Farooque 6 ; Ishfaq, Muhammad 7 ; Lakho, Shakeel Ahmed 8 ; Farooque, Muhammad 9 ; Shoaib, Muhammad 10 ; Zhang, Xiuying 1 ;

作者机构： 1.Northeast Agr Univ, Coll Vet Med, Fac Basic Vet Sci, Heilongjiang Key Lab Anim Dis Control & Pharmaceut, Harbin, Peoples R China

2.Shaheed Benazir Bhutto Univ Vet & Anim Sci, Fac Biosci, Dept Vet Pharmacol & Toxicol, Sakrand, Pakistan

3.Tech Ctr Chengdu Customs, Chengdu, Peoples R China

4.Zhejiang A&F Univ, Coll Anim Sci & Technol, Hangzhou, Peoples R China

5.Zhejiang A&F Univ, Coll Vet Med, Hangzhou, Peoples R China

6.Shaheed Benazir Bhutto Univ Vet & Anim Sci, Fac Vet Sci, Dept Vet Pathol, Sakrand, Pakistan

7.Huanggang Normal Univ, Huanggang, Peoples R China

8.Shaheed Benazir Bhutto Univ Vet & Anim Sci, Fac Vet Sci, Dept Vet Parasitol, Sakrand, Pakistan

9.Ziauddin Univ Karachi, Fac Vet & Anim Sci, Karachi, Pakistan

10.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab New Anim Drug Project,Minist Agr & Rural A, Gansu Prov Key Lab Vet Pharmaceut Dev, Lanzhou, Peoples R China

关键词： artesunate; babesiosis; histopathology; blood-borne pathogen; apoptosis; immune response; inflammation

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.2； 五年影响因子：3.5 ）

ISSN：

年卷期： 2024 年 11 卷

页码：

收录情况： SCI

摘要： Babesiosis, a zoonotic blood protozoal disease, threatens humans and animals and is difficult to treat due to growing antimicrobial resistance. The study aimed to investigate the therapeutic efficacy of artesunate (AS), a well-known derivative of artemisinin, against Babesia microti (B. microti) using a murine infection model. Male BALB/c mice (6 weeks old; 15 per group) were chosen and randomly divided into 1) the control group, 2) the B. microti group, and 3) the B. microti + artesunate treatment groups. AS treatment at 2 mg/kg, 4 mg/kg, and 8 mg/kg of body weight significantly (p < 0.05) reduced the B. microti load in blood smears in a dose-dependent manner. Additionally, AS treatment mitigated the decrease in body weight and restored the normal state of the liver and spleen viscera index compared to the B. microti-infected group after 28 days. Hematological analysis revealed significant increases in RBC, WBC, and PLT counts post-AS treatment compared to the B. microti-infected group. Furthermore, AS administration resulted in significant reductions in total protein, bilirubin, ALT, AST, and ALP levels, along with reduced liver and spleen inflammation and lesions as observed through histopathological analysis. AS also elicited dose-dependent changes in mRNA and protein expression levels of apoptotic, proinflammatory, and anti-inflammatory cytokines in the liver compared to the control and B. microti-infected groups. Immunolabeling revealed decreased expression of apoptotic and inflammation-related proteins in AS-treated hepatic cytoplasm compared to the B. microti-infected group. AS also in dose-dependent manner decreased apoptotic protein and increased Bcl-2. Overall, these findings underscore the potential of AS as an anti-parasitic candidate in combating B. microti pathogenesis in an in vivo infection model, suggesting its promise for clinical trials as a treatment for babesiosis.

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