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Molecular characterization, expression, and functional identification of TANK-binding kinase 1 (TBK1) of the cow (Bos taurus) and goat (Capra hircus)

文献类型： 外文期刊

作者： Yang, Xue 1 ; Ren, Shanhui 1 ; Rehman, Zaib Ur 3 ; Wang, Xiangwei 1 ; Yin, Xiangping 1 ; Sun, Yuefeng 1 ; Wan, Xuerui 2 ; Chen, Haotai 2 ;

作者机构： 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 733046, Peoples R China

2.Gansu Agr Univ, Coll Vet Med, Lab Vet Microbiol, Lanzhou 730070, Peoples R China

3.PMAS Arid Agr Univ, Fac Vet & Anim Sci, Dept Poultry Sci, Rawalpindi 46300, Pakistan

关键词： Cloning and expression; TBK1; Cow and goat; Antiviral activity; Innate immunity

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.605； 五年影响因子：3.833 ）

ISSN： 0145-305X

年卷期： 2022 年 133 卷

页码：

收录情况： SCI

摘要： The role of TANK-binding kinase 1 (TBK1) of humans and mice in innate immunity is well elucidated. Still, the molecular characterization and biological function of the TBK1 gene in herbivorous animals are less studied. Here, the open reading frame (ORF) of TBK1 of the cow and goat was firstly cloned and successfully expressed. The Phylogenetic tree analysis reveals that the TBK1 gene of goats and cows is similar to chicken and mute swans, respectively. Some evolutionary distances of the TBK1 gene were still present among different species. A slightly subcellular distribution difference was observed among full-length and truncated TBK1 of goats and cows. Dual-luciferase reporter assay has shown that the full-length TBK1 of goats and cows plays a vital role in the induction of IFN-8 production. The viral infection experiment showed that the over-expression of the fulllength TBK1 gene of the cow and goat significantly suppresses intracellular viral replication of the Lumpy skin disease virus (LSDV) in infected cells. Our study showed that TBK1 in the cows and goats is a crucial immunoregulatory for IFN-8 production during viral infection, contributing to a better understanding of innate immunity in the herbivorous animal.

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