Taraxacum mongolicum Hand.-Mazz. derived extracellular vesicles alleviate mastitis via NLRP3 inflammasome and NF-κB/MAPK pathways
文献类型： 外文期刊
作者： Sun, Yuan 1 ; Liu, Ying 1 ; Li, Jinxian 1 ; Huang, Shan 2 ; Du, Yiyang 1 ; Chen, Danyang 1 ; Yang, Min 1 ; Peng, Yinghua 1 ;
作者机构： 1.Chinese Acad Agr Sci, Jilin Prov Int Cooperat Key Lab Sci & Technol Inno, Inst Special Anim & Plant Sci, Changchun 130112, Jilin, Peoples R China
2.Changchun Univ Technol, Sch Chem & Life Sci, Changchun 130012, Jilin, Peoples R China
期刊名称：JOURNAL OF MATERIALS CHEMISTRY B （ 影响因子：5.7； 五年影响因子：6.1 ）
ISSN： 2050-750X
年卷期： 2025 年 13 卷 34 期
页码：
收录情况： SCI
摘要： Mastitis, a prevalent inflammatory disease affecting both humans and animals, imposes significant health burdens globally. Taraxacum mongolicum Hand.-Mazz. has been traditionally used to treat mammary gland disorders, however, the clinical translation of its crude extracts remains challenging due to the poor bioavailability. Emerging as innovative nanotherapeutic agents, plant-derived extracellular vesicles (PEVs) exhibit enhanced bioavailability, low immunogenicity, and targeted delivery capabilities, making them promising candidates for precision medicine applications. Herein, extracellular vesicles derived from Taraxacum mongolicum Hand.-Mazz. (TH-EVs) were successfully isolated employing ultracentrifugation and sucrose gradient centrifugation. Subsequently, these physicochemical properties, including particle size distribution and composition analysis, were comprehensively characterized. The anti-inflammatory efficacy and mechanism of TH-EVs were explored in both lipopolysaccharide (LPS)-stimulated murine mammary epithelial cells (HC11) and a murine mastitis model. In vitro, TH-EVs reduced TNF-alpha, IL-6, IL-1 beta and cellular oxidative stress. In vivo, TH-EVs alleviated histopathological damage, decreased myeloperoxidase activity, inhibited T lymphocyte activation, and reduced oxidative stress in mammary tissues. Mechanistically, TH-EVs inhibited the NLRP3 inflammasome, NF-kappa B and MAPK pathways. This study demonstrates that TH-EVs are a potent natural nanotherapeutic agent for mastitis, with potential for translational applications.
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