您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Metagenomic investigation of bacterial laccases in a straw-amended soil

文献类型： 外文期刊

作者： Yu, Dali 1 ; Liu, Ying 1 ; Cai, Hongying 2 ; Huang, Wanqiu 2 ; Wu, Huijun 3 ; Yang, Peilong 2 ;

作者机构： 1.Qilu Normal Univ, Jinan, Shandong, Peoples R China

2.Chinese Acad Agr Sci, Natl Engn Res Ctr Biol Feed, Minist Agr & Rural Affairs, Key Lab Feed Biotechnol,Inst Feed Res, Beijing, Peoples R China

3.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing, Peoples R China

关键词： Bacterial laccase; Metagenomics; Straw-amended soil; Profile hidden Markov models

期刊名称：PEERJ （ 影响因子：2.4； 五年影响因子：2.7 ）

ISSN： 2167-8359

年卷期： 2025 年 13 卷

页码：

收录情况： SCI

摘要： Background. Bacterial laccases play a crucial role in the degradation of lignin and the turnover of soil organic matter. Their advantageous properties make them highly suitable for a wide range of industrial applications. However, the limited identification of these potential enzymes has impeded their full utilization. The straw-amended soil provides materials for the development of bacterial laccases. Methods. Metagenomic sequencing of a straw-amended soil was conducted to explore novel bacterial laccases. The putative bacterial laccases were then screened using profile hidden Markov models for further analysis. The most abundant gene, lacs1, was heterologously expressed in Escherichia coli and the recombinant laccase was purified for enzymatic characterization. Results. A total of 322 putative bacterial laccases were identified in the straw-amended soil. Among them, 45 sequences had less than 30% identity to any entries in the Carbohydrate-Active Enzyme database and only 4.66% were more than 75% similar to proteins in the NCBI environmental database, exhibiting their novelty. These enzymes were found across various bacterial orders, demonstrating substantial diversity. Phylogenetic analysis revealed a number of the bacterial laccase sequences clustered with homologs characterized by favorable enzymatic properties. Five full-length representative bacterial laccase genes were obtained by modified thermal asymmetric interlaced PCR. The laccase activity of lacS1 was validated. It was a mesophilic enzyme with alkaline stability and halotolerance, indicating its promise for industrial applications. Implications. These findings highlight novel bacterial laccase resources with potential for industrial applications and enzyme engineering.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台