文献类型： 外文期刊

作者： Ullah, Ikram ¹ ; Uzair, Muhammad ² ; Rehman, Obaid Ur ² ; Attacha, Safira ³ ; Attia, Kotb A. ⁴ ; Ahmad, Sheraz ⁵ ; Salman, Muhammad ⁶ ; Ali, Hamid ⁷ ; Rashid, Muhammad Abdul Rehman ⁸ ; Abushady, Asmaa M. ⁹ ; Fiaz, Sajid ¹¹ ; Shah, Syed Jehangir ³ ; Kimiko, Itoh ¹² ; Chen, Rugang ¹³ ; Liu, Jiaxun ¹⁴ ; Wu, Hongzhi ¹ ;

作者机构： 1.Yunnan Agr Univ, Coll Landscape & Hort, Kunming 650201, Peoples R China

2.Natl Inst Genom & Adv Biotechnol NIGAB, Islamabad 45500, Pakistan

3.Univ Agr, Inst Biotechnol & Genet Engn, Peshawar, Pakistan

4.King Saud Univ, Coll Sci, Dept Biochem, Riyadh 11451, Saudi Arabia

5.Yangzhou Univ, Sch Hort & Plant Protect, Yangzhou 225009, Peoples R China

6.Ghazi Univ, Dept Hort, Dera Ghazi Khan, Pakistan

7.Univ Agr Peshawar, Fac Crop Prod Sci, Dept Hort, Peshawar, Pakistan

8.Govt Coll Univ, Coll Agr & Environm Sci, Dept Agr Sci, Faisalabad 38000, Pakistan

9.Nile Univ, Biotechnol Sch, 26th July Corridor, Sheikh Zayed City 12588, Giza, Egypt

10.Ain Shams Univ, Fac Agr, Dept Genet, Cairo, Egypt

11.Univ Haripur, Dept Plant Breeding & Genet, Haripur 22620, Pakistan

12.Niigata Univ, Inst Sci & Technol, Ikarashi 2, Niigata, Niigata 9502181, Japan

13.Northwest A&F Univ, Coll Hort, Yangling 712100, Peoples R China

14.Yunnan Acad Agr Sci, Inst Hort Res, Kunming, Yunnan, Peoples R China

关键词： A-biotic stress; Cucumber; CsTLPs; Genome-wide; Gene ontology analysis

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：2.0； 五年影响因子：1.9 ）

ISSN： 0925-9864

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Cucumber (Cucumis sativus L.) is a member of the Cucurbitaceae family and can be eaten raw in salads or cooked as a vegetable. The yield of cucumber is limited due to many factors including biotic and abiotic stresses. Tubby-like proteins (TLP) family genes are important regulators of growth and development in plants and are involved in a variety of biotic and abiotic stress responses. The specific function of the TLP family in cucumber has not been thoroughly investigated by a genetic study. Hence, we performed a comparative genomic analysis of cucumber TLP family genes using bioinformatics techniques. Herein, a total of 11 TLP proteins were identified from the cucumber genome and further divided into four different subgroups. Moreover, these CsTLPs were distributed randomly on the five chromosomal positions of cucumber. Motif analysis suggested that motif1 was commonly present in all CsTLPs proteins except CsTLP6. Gene ontology analysis of CsTLPs showed their involvement in the plasma membrane, cellular protein modifications, signal transduction, and stress responses. Similarly, Cis-acting elements showed stress-related elements, validating their role in mitigating various stresses. Expression profiling indicated that CsTLPs expressed dominantly with diverse expression patterns in various organs of the cucumber. The genes CsTLP2, CsTLP5, CsTLP7, and CsTLP10 showed higher expression as compared to the rest of the CsTLPs members under salt stress. The above findings and details can serve as a starting point for further research on TLP function in plants, especially in cucumbers.