Combining Transcriptome Analysis and Comparative Genomics to Identify Key Components of the Lignin Biosynthesis Gene Network in Sorghum bicolor
文献类型： 外文期刊
作者： Niu, Hao 1 ; Wang, Yanbo 3 ; Liu, Ruizhen 4 ; Cheng, Xiaoqiang 4 ; Wang, Yao 4 ; Wang, Yubin 1 ; Lv, Xin 1 ; Fan, Fangfang 1 ; Ju, Lan 1 ; Chu, Jianqiang 1 ; Yan, Haisheng 1 ; Wang, Hongru 3 ; Chang, Hetan 3 ; Zhang, Yancong 3 ; Tao, Yongfu 3 ; Ping, Junai 1 ;
作者机构： 1.Shanxi Agr Univ, Inst Sorghum, Jinzhong 030600, Peoples R China
2.Shanxi Hou Ji Lab, Taiyuan 030031, Peoples R China
3.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Guangdong Lab Lingnan Modern Agr, Key Lab Synthet Biol,Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
4.Shanxi Agr Univ, Coll Agr, Jinzhong 030801, Peoples R China
关键词： sorghum; lignin content; transcriptomics; differentially expressed genes; functional enrichment; sequence variation
期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）
ISSN：
年卷期： 2025 年 15 卷 7 期
页码：
收录情况： SCI
摘要： Sorghum is a versatile crop that serves as a major source of food, feed, fodder and biofuel globally. Lignin content in sorghum affects multiple important traits, including lodging resistance, forage digestibility and the efficiency of bioenergy production. However, the genetic regulation of lignin content in sorghum remains poorly understood. In this study, we combined transcriptomic and comparative genomic approaches to uncover the genetic network underlying lignin biosynthesis in sorghum. Through comparative genomic analysis, we identified 104 candidate genes involved in lignin biosynthesis. Transcriptome analysis of four sorghum accessions with contrasting lignin contents identified 6132 differentially expressed genes with an enrichment of genes related to phenylpropanoid biosynthesis and cell wall biogenesis. The 104 lignin biosynthesis candidates were significantly enriched (p-value < 0.01) in these differentially expressed genes, with most differentially expressed candidate genes related to monolignol biosynthesis and polymerization being up-regulated in high-lignin accessions. These up-regulated genes are related to all key enzymes involved in lignin biosynthesis, suggesting that the elevated lignin content in these accessions results from a collective increase in enzyme activity. Sequence analysis revealed a significant reduction in genetic diversity across lignin biosynthesis genes in cultivated sorghum compared to wild sorghum. Moreover, selection signals during domestication were identified in 30 lignin biosynthesis genes, 22 of which were differentially expressed, further supporting the functional relevance of these differentially expressed genes in lignin biosynthesis. Overall, our findings uncover the lignin biosynthesis gene network in sorghum and offer potential targets for future functional studies and trait manipulation.
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