文献类型： 外文期刊

作者： Labroo, Marlee R. ¹ ; Clark, Lindsay V. ¹ ; Zhang, Shilai ² ; Hu, Fengyi ² ; Tao, Dayun ³ ; Hamilton, Ruaraidh Sackville ⁵ ; Sacks, Erik J. ¹ ;

作者机构： 1.Univ Illinois Champaign Urbana, Dept Crop Sci, Urbana, IL 61801 USA

2.Yunnan Univ, Res Ctr Perennial Rice Engn & Technol Yunnan, Sch Agr, Kunming 650091, Yunnan, Peoples R China

3.Yunnan Acad Agr Sci YAAS, Food Crops Res Inst, Yunnan Seed Lab, Kunming, Peoples R China

4.Yunnan Acad Agr Sci YAAS, Food Crops Res Inst, Yunnan Key Lab Rice Genet Improvement, Kunming, Peoples R China

5.Int Rice Res Inst, TT Chang Genet Resources Ctr, Los Banos, Philippines

6.CGIAR Genebank Initiat, Salisbury, England

关键词： Oryza longistaminata; Oryza sativa (domesticated Asian rice); Oryza glaberrima (domesticated African rice); interspecific hybridization; crop wild relatives (CWR); AA genome; germplasm conservation; population genetics

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： The undomesticated rice relative Oryza longistaminata is a valuable genetic resource for the improvement of the domesticated Asian rice, Oryza sativa. To facilitate the conservation, management, and use of O. longistaminata germplasm, we sought to quantify the population structure and diversity of this species across its geographic range, which includes most of sub-Saharan Africa, and to determine phylogenetic relationships to other AA-genome species of rice present in Africa, including the prevalence of interspecific hybridization between O. longistaminata and O. sativa. Though past plant breeding efforts to introgress genes from O. longistaminata have improved biotic stress resistance, ratooning ability, and yield in O. sativa, progress has been limited by substantial breeding barriers. Nevertheless, despite the strong breeding barriers observed by plant breeders who have attempted this interspecific cross, there have been multiple reports of spontaneous hybrids of O. sativa and O. longistaminata (aka "Obake") obtained from natural populations in Africa. However, the frequency and extent of such natural introgressions and their effect on the evolution of O. longistaminata had not been previously investigated. We studied 190 O. longistaminata accessions, primarily from the International Rice Research Institute genebank collection, along with 309 O. sativa, 25 Oryza barthii, and 83 Oryza glaberrima control outgroups, and 17 control interspecific O. sativa/O. longistaminata hybrids. We analyzed the materials using 178,651 single-nucleotide polymorphisms (SNPs) and seven plastid microsatellite markers. This study identified three genetic subpopulations of O. longistaminata, which correspond geographically to Northwestern Africa, Pan-Africa, and Southern Africa. We confirmed that O. longistaminata is, perhaps counterintuitively, more closely related to the Asian species, O. sativa, than the African species O. barthii and O. glaberrima. We identified 19 recent spontaneous interspecific hybrid individuals between O. sativa and O. longistaminata in the germplasm sampled. Notably, the recent introgression between O. sativa and O. longistaminata has been bidirectional. Moreover, low levels of O. sativa alleles admixed in many predominantly O. longistaminata accessions suggest that introgression also occurred in the distant past, but only in Southern Africa.