PIN1a-mediated auxin release from rootstock cotyledon contributes to healing in watermelon as revealed by seeds soaking-VIGS and cotyledon grafting
文献类型： 外文期刊
作者： Wang, Xiao 1 ; Xiong, Mu 1 ; Xu, Jianuo 1 ; Zhang, Ting 1 ; Kadeer, Akebaierjiang 1 ; Bie, Zhilong 1 ; Notaguchi, Michitaka 1 ; Huang, Yuan 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Natl Key Lab Germplasm Innovat & Utilizat Crops, Wuhan 430070, Peoples R China
2.Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan
3.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Shenzhen 518100, Peoples R China
4.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr, Shenzhen 518100, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）
ISSN： 2662-6810
年卷期： 2025 年 12 卷 3 期
页码：
收录情况： SCI
摘要： Grafting is a propagation method extensively utilized in cucurbits. However, the mechanisms underlying graft healing remain poorly understood. This study employed self-grafted watermelon plants to investigate how rootstock cotyledon affects healing. The complete removal of rootstock cotyledons significantly hindered scion growth, as evidenced by reductions in scion fresh weight and the area of true leaves. Physiological assessments revealed reduced callus formation, weaker adhesion forces, a more pronounced necrotic layer, and decreased rates of xylem and phloem reconnection at the graft junction when rootstock cotyledons were completely removed. Additionally, auxin levels at the rootstock graft junction notably decreased following cotyledon removal. In contrast, the exogenous application of indole-3-acetic acid (IAA) notably enhanced graft healing. Moreover, gene expression analysis of the PIN auxin efflux carriers in the rootstock cotyledons indicated significant activation of ClPIN1a postgrafting. Furthermore, we developed an improved Virus-Induced Gene Silencing (VIGS) system for cucurbits using seeds soaking method. This method achieved an infection success rate of 83% with 60%-75% gene silencing efficiency, compared to the 37% success rate with 40%-60% efficiency seen with traditional cotyledon infection. Combining our novel VIGS approach with cotyledon grafting techniques, we demonstrated that rootstock cotyledons regulate callus formation through ClPIN1a-mediated endogenous auxin release, thus facilitating graft union development. These findings suggest potential strategies for enhancing watermelon graft healing by manipulating rootstock cotyledons.
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