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Full-length transcriptome sequencing of the longissimus dorsi muscle of yak and cattle-yak using nanopore technology

文献类型： 外文期刊

作者： Cao, Mengli 1 ; Ding, Ziqiang 1 ; Wang, Xingdong 3 ; Guo, Shaoke 1 ; Kang, Yandong 1 ; Hu, Liyan 1 ; Zhang, Ben 1 ; Pei, Jie 1 ; Ma, Yi 4 ; Guo, Xian 1 ;

作者机构： 1.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Yak Breeding Gansu Prov, Lanzhou 730050, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Anim Genet & Breeding Tibetan Plateau, Lanzhou 730050, Peoples R China

3.Inner Mongolia Normal Univ, Coll Life Sci & Technol, Hohhot 010022, Peoples R China

4.Tianjin Acad Agr Sci, Tianjin 300192, Peoples R China

关键词： Yak; Cattle-yak; Longissimus dorsi; Alternative splicing; Full-length transcriptom

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 284 卷

页码：

收录情况： SCI

摘要： Short-read RNA sequencing has been used to sequence the transcriptome of the skeletal muscle of yak and cattle- yak; however, full-length transcripts cannot be obtained and alternative splicing (AS) events cannot be inferred using this sequencing approach. Here, we used Oxford Nanopore Technologies (ONT) full-length sequencing to sequence the transcriptome of the longissimus dorsi of yak and cattle-yak. A total of 20,323 novel genes and 172,870 novel transcripts were identified, and 159,700 novel transcripts were successfully annotated. A total of 157,812 AS events, 58,073 simple sequence repeats, 57,468 complete open reading frames, 2296 transcription factors, and 20,404 lncRNAs were detected. Differentially expressed transcripts (DETs) in the longissimus dorsi muscle of yak and cattle-yak were involved in the MAPK and JAK-STAT signaling pathways related to muscle development and growth. Protein-protein interaction analysis of DETs suggested that TNNI2 might make a major contribution to differences in muscle growth and meat quality traits between yak and cattle-yak. The results have enriched the transcriptome data of dorsal muscles, providing new ideas for the study of transcriptional regulation processes, and also providing useful information for the production of higher yields of yak meat.

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