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Expression of mitogen-activated protein kinase double-stranded RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea

文献类型： 外文期刊

作者： Chen Guo-hua 1 ; Tian Xue-liang 2 ; Wang Dian-dong 3 ; Ling Jian 1 ; Mao Zhen-chuan 1 ; Yang Yu-hong 1 ; Xie Bing-yan 1 ;

作者机构： 1.Chinese Acad Agr Sci, Minist Agr, Inst Vegetable & Flowers, Key Lab Hort Crops Genet Improvement, Beijing 100081, Peoples R China

2.Henan Inst Sci & Technol, Coll Resources & Environm, Xinxiang 453003, Peoples R China

3.Yangtze Normal Univ, Life Sci & Technol Inst, Fuling 408100, Peoples R China

关键词： cucumber;16S rDNA;diversity;rhizosphere archaea;mapk dsRNA

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 10 期

页码：

收录情况： SCI

摘要： The expression of mitogen-activated protein kinase (mapk) double-stranded RNA in cucumber is effective in controlling infestations of the root-knot nematode Meloidogyne incognita. However, little is known about the ecological effects of transgenic plants. Here, we analyzed the diversity of 16S rDNA genes derived from the rhizosphere archaea of transgenic cucumber plants as an indicator of ecological change. A total of 17 and 18 operational taxonomic units were detected in the rhizospheres of non-transgenic cucumber and mapk dsRNA-expressing plants, respectively. No significant difference was observed between the two groups according to Shannon and Simpson indices. In soil samples of the two rhizospheres, the dominant group was Crenarchaeota at the phylum level, with Staphylothermus, Methanococcus, Pyrodictium and Sulfolobus the abundant taxa at the genus level. These results suggest that expressing mapk double-stranded (ds) RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea, and provide powerful evidence for the ecological safety of transgenic cucumber expressing mapk dsRNA.

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