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A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks

文献类型: 外文期刊

作者: Bai Miao 1 ; Chen Wen-ting 1 ; Xie Bing-yan 2 ; Yang Gun-shun 1 ;

作者机构: 1.Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

关键词: Cucumber mosaic virus (CMV);graft;RNA silencing;Solanum lycopersicum;virus resistance

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2016 年 15 卷 9 期

页码:

收录情况: SCI

摘要: Cucumber mosaic virus (CMV) can infect a wide range of host species. For the lacking of CMV resistant varieties of tomato, RNA interference (RNAi) can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA (ihpRNA) plant expression vectors aimed at five genes of CMV have been constructed. Transgenic tomatoes were obtained by Agrobacterium tumefaciens-mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism (GMO) biosafety.

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