您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Monoclonal antibody and B-cell epitope mapping of the VP7 protein in bluetongue virus

文献类型： 外文期刊

作者： Hu, Xin-Bing 1 ; Xu, Jing 1 ; Zhang, Yu 1 ; Meng, Fan-Hua 1 ; Tian, Zhan-Cheng 1 ; Guan, Gui-Quan 1 ; Luo, Jian-Xun 1 ; Yin, Hong 1 ; Du, Jun-Zheng 1 ;

作者机构： 1.Lanzhou Univ, Chinese Acad Agr Sci, State Key Lab Anim Dis Control & Prevent, Coll Vet Med,Lanzhou Vet Res Inst, Lanzhou 730000, Peoples R China

2.Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Lanzhou 730046, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China

关键词： Bluetongue virus; VP7 protein; Monoclonal antibodies; B-cell epitope mapping

期刊名称：VIROLOGY JOURNAL （ 影响因子：3.8； 五年影响因子：3.7 ）

ISSN：

年卷期： 2025 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Bluetongue virus (BTV) VP7 is a group-specific protein that is highly conserved in different serotypes. In this study, BALB/c mice were immunized with purified recombinant BTV-1 VP7 protein expressed in E. coli. Then six monoclonal antibodies (mAbs), 2A7, 2B2, 2B3, 2D3, 2D7, and 2H2, against the BTV-1 VP7 protein were produced using hybridoma technology. The reactivity of the mAbs was identified using western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assay. A series of truncated peptides derived from VP7 expressed as glutathione S-transferase fusion proteins were mapped with mAbs by western blotting. The results indicated that 2A7 recognized the epitope 71SAAGINVGPI80, 2B3 recognized 110ARVTGETSTWG120, 2B2 and 2D3 recognized 125PYGFFLETEET135, and 2D7 and 2H2 recognized 332VNPMPGPLTRA342. Amino acid sequence analysis showed that these four epitopes were conserved in 24 typical BTV serotypes. Cross-reaction results showed that mAb 2A7 could recognize the recombinant VP7 protein of BTV-1, African horse sickness virus serotype 1 (AHSV-1), and epidemic hemorrhagic disease virus serotype 1 (EHDV-1). The mAbs 2B2, 2B3, and 2D3 could recognize the recombinant VP7 protein of BTV-1 and EHDV-1, and the mAbs 2D7 and 2H2 specifically recognized the BTV-1 VP7 protein. These specific mAbs and identified B-cell epitopes provided key insights into the structure and function of VP7, while facilitating the development of BTV diagnostics and the design of epitope-based vaccines.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台