Complete nucleotide sequence of pBMB67, a 67-kb plasmid from Bacillus thuringiensis strain YBT-1520
文献类型: 外文期刊
作者: Liu Chao 1 ; Bao Qiyu 1 ; Song Fuping 1 ; Sun Ming 2 ; Huang Dafang; Liu Guiming; Yu Ziniu;
作者机构: 1.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China; Huazhong Agr Univ, Natl Engn Ctr Microbe Pesticides, Wuhan 430070, Peoples R China; Zhejiang Univ, James D Watson Inst Genome Sci, Hangzhou 310008, Peoples R China; Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China; Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100094, Peoples R China
2.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peo
关键词: Bacillus thuringiensis;cryptic plasmid;complete nucleotide sequence
期刊名称:PLASMID ( 影响因子:3.466; 五年影响因子:2.74 )
ISSN: 0147-619X
年卷期: 2007 年 57 卷 1 期
页码:
收录情况: SCI
摘要: The complete nucleotide sequence of a large (67 kb) cryptic plasmid pBMB67 from Bacillus thuringiensis strain YBT-1520 was determined. Of the 74 predicted open reading frames (ORFs), 25 (34%) were assigned putative functions, 18 (24%) encoded conserved hypothetical proteins, and 31 (42%) had no homology to any genes present in the current open databases. The ORFs with similar functions were organized in a modular structure; thus, the DNA sequence of pBMB67 could be functionally divided into three modules, including a 39 kb transfer region encoding homologs of the Agrobacterium tumefaciens VirB/D4 system components VirB1, VirB4, VirB11, and VirD4, as well as homologs of Gram-positive conjugation proteins. We also found a potential operon that was analogous to the Rap-Phr cassettes from Bacillus subtilis, which are involved in cell-cell communication and transcriptional regulation. Thus, we suggest that pBMB67 is likely to be implicated in cell-cell signaling and plays a role in the regulation of several cellular processes, with the production of exoprotease being one of the candidates. (c) 2006 Elsevier Inc. All rights reserved.
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