您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

In vivo fluorescence observation of parasporal inclusion formation in Bacillus thuringiensis

文献类型： 外文期刊

作者： Yang Hui 2 ; Rong Rong 1 ; Song FuPing 1 ; Sun ChangPo 1 ; Wei Juan 1 ; Zhang Jie 1 ; Huang DaFang 2 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词： Bacillus thuringiensis;CrylAc-GFP fusion protein;laser confocal microscopy

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

年卷期： 2010 年 53 卷 9 期

页码：

收录情况： SCI

摘要： A recombinant gene expressing a CrylAc-GFP fusion protein with a molecular mass of approximately 160 kD was constructed to investigate the expression of crylAc, the localization of its gene product CrylAc, and its role in crystal development in Bacillus thuringiensis. The crylAc-gfp fusion gene under the control of the crylAc promoter was cloned into the plasmid pHT304, and this construct was designated pHTcrylAc-gfp. pHTcrylAc-gfp was transformed into the crystal-negative strain, HD-73 cry(-), and the resulting strain was named HD-73(-)(pHTcrylAc-gfp). The gfp gene was then inserted into the large HD-73 endogenous plasmid pHT73 and fused with the 3' terminal of the ctylAc gene by homologous recombination, yielding HD-730 Phi(crylAc-gfp)3534. Laser confocal microscopy and Western blot analyses showed for the first time that the CrylAc-GFP fusion proteins in both HD-73(-)(pHTcrylAc-gfp) and HD-730 Phi(crylAc-gfp)3534 were produced during asymmetric septum formation. Surprisingly, the CrylAc-GFP fusion protein showed polarity and was located near the septa in both strains. There was no significant difference between CrylAc-GFP and CrylAc in their toxicity to Plutella xylostella larvae.

  • 相关文献

[1]Resistance alleviation in the larval cotton bollworm to fenvalerate after pretreatment with bacillus thuringiensis. 谭维嘉,梁革梅,郭予元. 1999

[2]利用ubi1内含子增强Bt cry1Ah基因在转基因玉米中的表达. 郎志宏,张杰,何康来,宋福平,黄大昉. 2008

[3]棉铃虫中肠氨肽酶APN4与Cry1Ac、Cry2Aa结合能力的比较. 葛朝虹,肖玉涛,梁革梅. 2015

[4]产Zwittermicin A的苏云金芽孢杆菌菌株的筛选及抗性基因zmaR的克隆与表达. 宋福平,李卓夫,张杰,姜声华,刘大群,黄大昉. 2005

[5]Research And Development of Recombinant Microbial Agents And Biosafety Consideration in China. Dafang Huang,International Society for Biosafety Ressarch; Peking University; Ministry of Science & Technology(MOST);. 2002

[6]棉铃虫、甜菜夜蛾中肠受体蛋白与Bt毒素结合能力的研究. 袁向东. 2016

[7]亚洲玉米螟对几种Bt蛋白的交互抗性及其中肠上结合位点的研究. 韩海亮. 2009

[8]Effect of pyramiding Bt and CpTI genes on resistance of cotton to Helicoverpa armigera (Lepidoptera: Noctuidae) under laboratory and field conditions. Jinjie Cui,Junyu Luo,Wopke Van Der Werf,Yan Ma,Jingyuan Xia.

[9]Identification of ABCC2 as a binding protein of Cry1Ac onbrush border membrane vesicles from Helicoverpa armigera by an improved pull-down assay. Wang, Zeyu,Zhou, Xueping,Zhou, Zishan,Liu, Yuxiao,Liang, Gemei,Shu, Changlong,Song, Fuping,Zhou, Xueping,Zhang, Jie,Bravo, Alejandra,Soberon, Mario. 2016

[10]Processing of delta-endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 in Heliothis armigera midgut juice and the effects of protease inhibitors. Shao, ZZ,Cui, YL,Liu, XL,Yi, HQ,Ji, JH,Yu, ZN. 1998

[11]Down-regulation of aminopeptidase N and ABC transporter subfamily G transcripts in Cry1Ab and Cry1Ac resistant Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). Wang, Yueqin,Wang, Yidong,Bai, Shuxiong,Wang, Zhenying,He, Kanglai,Coates, Brad S.. 2017

[12]Efficacy of transgenic cotton containing a cry1Ac gene from Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera : Noctuidae) in Northern China. Guo, YY,Lv, N,Greenplate, JT,Deaton, R. 2003

[13]Monitoring of resistance for the diamondback moth to Bacillus thuringiensis Cry1Ac and Cry1Ba toxins and a Bt commercial formulation. Li, X.-F.,Zhang, J.,Zhao, J.-Z.,Wu, Q.-J.,Xu, B.,Zhang, Y.-J.. 2007

[14]Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela. Wei, Lei,Wu, Xiaoai,Rao, Zihe,Guo, Shuyuan,Guo, Shuyuan,Liu, Yanfeng,Xue, Jing,Wu, Hongfu,Song, Fuping,Zhang, Jie,Huang, Dafang. 2009

[15]Development of Bt Rice and Bt Maize in China and Their Efficacy in Target Pest Control. Liu, Qingsong,Peng, Yufa,Li, Yunhe,Hallerman, Eric. 2016

[16]Impacts of transgenic cry1Ab rice on non-target planthoppers and their main predator Cyrtorhinus lividipennis (Hemiptera : Miridae) - A case study of the compatibility of Bt rice with biological control. Liu, Zhi-Cheng,Ye, Gong-yin,Shen, Zhi-cheng,Hu, Cui,Peng, Yu-fa,Altosaar, Illimar,Shelton, Anthony M.. 2007

[17]Assembling of Holotrichia parallela (dark black chafer) midgut tissue transcriptome and identification of midgut proteins that bind to Cry8Ea toxin from Bacillus thuringiensis. Shu, Changlong,Tan, Shuqian,Yin, Jiao,Geng, Lili,Song, Fuping,Li, Kebin,Zhang, Jie,Soberon, Mario,Bravo, Alejandra,Liu, Chunqing.

[18]Bt Toxin Cry1Ie Causes No Negative Effects on Survival, Pollen Consumption, or Olfactory Learning in Worker Honey Bees (Hymenoptera: Apidae). Diao, Qing-Yun,Jia, Hui-Ru,Geng, Li-Li.

[19]A genetically modified broad-spectrum strain of Bacillus thuringiensis toxic against Holotrichia parallela, Anomala corpulenta and Holotrichia oblita. Zhao, Can,Shu, Changlong,Song, Fuping,Zhang, Jie,Wang, Qinglei,Feng, Xiaojie.

[20]Identification of the minimal active fragment of the Cry1Ah toxin. Zhou, Zishan,Song, Fuping,Shu, Changlong,Zhang, Jie,Huang, Dafang.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台