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Cloning of fatty acid elongase 1 gene and molecular identification of A and C genome in Brassica species

文献类型: 外文期刊

作者: Wu Yuhua 1 ; Xiao Ling 1 ; Wu Gang 1 ; Lu ChangMing 1 ;

作者机构: 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan, Peoples R China

关键词: brassica;fatty acid elongase 1;A and C genome identification;AvrII

期刊名称:SCIENCE IN CHINA SERIES C-LIFE SCIENCES ( 影响因子:1.61; 五年影响因子:1.148 )

ISSN: 1006-9305

年卷期: 2007 年 50 卷 3 期

页码:

收录情况: SCI

摘要: The fatty acid elongase 1 (FAE1) genes of Brassic napus were cloned from two cultivars, i.e. Zhongshuan No. 9 with low erucic acid content, and Zhongyou 821 with high erucic acid content, using the degenerate PCR primers. The sequence analysis showed that there was no intron within the FAE1 genes. The FAE1 genes from Zhongyou 821 contained a coding sequence of 1521 nucleotides, and those cloned from ZhongE;huan No. 9 contained a 1517 bp coding sequence. Alignment of the FAE1 sequences from Brassica rapa, B. oleracea and B. napus detected 31 single nucleotide polymorphic sites (2.03%), which resulted in 7 amino-acid substitutions. Further analysis indicated that 19 SNPs were genome-specific, of which, 95% were synonymous mutations. The nucleotide substitution at position 1217 in the FAE1 genes led to a specific site of restricted cleavage. An AvrII cleavage site was present only in the C genome genes and absent in the A genome FAE1 genes. Digestion profile of the FAE1 sequences from B. rapa, B. oleracea and B. napus produced with AvrII confirmed that the FAE1 genes of B. oleracea origin was recognized and digested, while that of B. rapa origin could not. The results indicated that by AvrII cleavage it was possible to distinguish B. rapa from B. oleracea and between the A and C genome of B. napus. In addition, the FAE1 genes could be used as marker genes to detect the pollen flow of P. napus, thus providing an alternative method for risk assessment of gene flow.

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