您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events

文献类型： 外文期刊

作者： Wu Yu-hua 1 ; Zhang Li 1 ; Wu Gang 1 ; Nie Shu-jing 1 ; Lu Chang-ming 1 ;

作者机构： 1.Chinese Acad Agr Sci, Oilcrops Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China

2.South Cent Univ Nationalities, Coll Life Sci, Wuhan 430074, Peoples R China

关键词： transgenic rapeseed;junction fragment;pre-insertion site;DNA rearrangement

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2014 年 13 卷 9 期

页码：

收录情况： SCI

摘要： To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration., During transformation in each of the six events, portions of both the right border (RB) and left border (LB) regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head (RB-to-RB) concatemer into the recipient genome. In event MS 1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.

  • 相关文献

[1]Event-specific qualitative and quantitative PCR detection methods for Transgenic rapeseed hybrids MS1 x RF1 and MS1 x RF2. Wu, Yuhua,Wu, Gang,Xiao, Ling,Lu, Changming. 2007

[2]On-line pre-concentration and UV determination of DNA fragments by dynamic coating capillary electrophoresis and its application to detection of genetically modified oilseed rape based on PCR. Chen, Hong,Wu, Yu-Hua,Song, Dan-Yang,Zhang, Wen,Dong, Xu-Yan,Li, Pei-Wu,Lu, Chang-Ming.

[3]Commercialization of Transgenic Rapeseed and Countermeasure Taken in Biosafety Management. Changming Lu,China Association of Agricultural Science Societies; Ministry of Agriculture, P.R.China; China Association for Science and Technology;. 2006

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台