您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cloning and Expression of Calcium-Dependent Protein Kinase (CDPK) Gene Family in Common Tobacco (Nicotiana tabacum)

文献类型： 外文期刊

作者： Tai Shuai-shuai 1 ; Liu Guan-shan 1 ; Sun Yu-he 1 ; Chen Jia 2 ;

作者机构： 1.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Qual Control, Minist Agr, Qingdao 266101, Peoples R China

2.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China

关键词： common tobacco (Nicotiana tabacum);CDPK;phylogenetic tree;gene expression

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2009 年 8 卷 12 期

页码：

收录情况： SCI

摘要： To further study the function of calcium-dependent protein kinase (CDPK) gene family in common tobacco (Nicotiana tabacum), it is necessary to isolate more CDPKs from common tobacco and describe the sequence characteristics, evolutionary relationship and gene expression. Reverse transcription-PCR (RT-PCR), rapid amplification of cDNA (RACE) and bioinformatics methods were used to isolate CDPKs from common tobacco. A phylogenetic tree was created using the MEGA4.0 program and expression patterns of the three full-length CDPK genes were studied by RT-PCR. After all aforementioned efforts, we obtained eight additional common tobacco CDPK genes, of which three possessed complete open reading frames (ORFs). Phylogenetic analysis divided 11 full-length Nicotiana CDPK genes into four subfamilies, and two putative common tobacco and Arabidopsis orthologous CDPK genes might correspond to well-conserved functions. Three full-length CDPK genes in common tobacco were detected in all tobacco organs tested, but their expression patterns were significantly different. Eight non-redundant common tobacco CDPK genes were isolated in this study. Along with the previously characterized CDPK genes, at least 15 members of the CDPK family exist in common tobacco. This work establishes a foundation for a genome-wide study of this important gene family in common tobacco.

  • 相关文献

[1]VfCPK1, a gene encoding calcium-dependent protein kinase from Vicia faba, is induced by drought and abscisic acid. Liu, Guanshan,Chen, Jia,Wang, Xuechen. 2006

[2]Molecular cloning and characterization of a novel stress responsive gene in alfalfa. Sun, Y.,Long, R.,Yang, Q.,Kang, J.,Chao, Y.,Wu, M.,Long, R.,Wang, P.,Qin, Z.. 2012

[3]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Hu Genhai,Yu Shuxun,Fan Shuli,Song Meizhen. 2007

[4]普通烟草钙依赖蛋白激酶NtCDPK15的基因克隆及表达分析. 康乐,张丽,张磊,吴清章,丁安明,宗鹏,刘贯山. 2013

[5]普通烟草CDPK基因家族的克隆及表达分析. 太帅帅,刘贯山,孙玉合,陈珈. 2009

[6]Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.). Li, Ai-Li,Zhu, Yuan-Fang,Tan, Xiao-Mei,Wang, Xiang,Wei, Bo,Guo, Han-Zi,Zhang, Zeng-Lin,Chen, Xiao-Bo,Zhao, Guang-Yao,Kong, Xiu-Ying,Jia, Ji-Zeng,Mao, Long,Tan, Xiao-Mei.

[7]Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum. Chen Shuai,Liu Guan-shan,Wang Yuan-ying,Sun Yu-he,Chen Jia. 2011

[8]Characterization of the ZmCK1 Gene Encoding a Calcium-Dependent Protein Kinase Responsive to Multiple Abiotic Stresses in Maize. Wang, Chang-Tao,Shao, Jun-Ming.

[9]烟草钙依赖蛋白激酶基因克隆与分析. 康乐. 2013

[10]Protein restriction followed by tea li men tat ion influenced immune system and transcriptome of lamb. K. Cm,B. Wang,N.F. Zhang. 2019

[11]Gene Expression Profile of Osteoblast-Like Cells on Calcium Phosphate Biomaterials. Taro Takemura,Hongsong Fan,Toshiyuki lkoma,Junzo Tanaka,Nobutaka Hanagata. 2007

[12]Effect of Acid Treated Hydroxyapatite on Osteoblast Maturation. Taro Takemura,Toshiyuki Ikoma,Nobutaka Hanagata. 2008

[13]Chondrocyte gene expression under applied surface motion. Sibylle Grad,Cynthia R. Lee,Markus A. Wimmer,Mauro Alin. 2006

[14]Genomic organization, differential expression, and functional analysis of the SPL gene family in Gossypium hirsutum. Zhang, Xiaohong,Dou, Lingling,Pang, Chaoyou,Song, Meizhen,Wei, Hengling,Fan, Shuli,Wang, Chengshe,Yu, Shuxun. 2015

[15]Genome-wide identification and functional analysis of the TIFY gene family in response to drought in cotton. Ge Zhao,Yun Song,Caixiang Wang,Hamama Islam Butt,Qianhua Wang,Chaojun Zhang,Zuoren Yang,Zhao Liu,Eryong Chen,Xueyan Zhang,Fuguang Li.

[16]Provirus variants of bovine leukemia virus in naturally infected cattle from Argentina and Japan. Licursi, M,Inoshima, Y,Wu, DL,Yokoyama, T,Gonzalez, ET,Sentsui, H. 2003

[17]Molecular diversity and phylogeny of rhizobia associated with Lablab purpureus (Linn.) grown in Southern China. Chang, Yue Li,Wang, En Tao,Sui, Xin Hua,Chen, Wen Xin,Wang, En Tao,Zhang, Xiao Xia.

[18]Genome-wide analysis of AP2/ERF transcription factor family in Zea mays. Zhou, Mei-Liang,Tang, Yi-Xiong,Wu, Yan-Min,Zhou, Mei-Liang.

[19]Molecular phylogeny of the domesticated silkworm, Bombyx mori, based on the sequences of mitochondrial cytochrome b genes. Li, AL,Zhao, QL,Tang, SM,Zhang, ZF,Pan, SY,Shen, GF.

[20]Genome-wide identification of genes involved in raffinose metabolism in Maize. Zhou, Mei-Liang,Zhang, Qian,Zhou, Ming,Shao, Ji-Rong,Zhou, Mei-Liang,Sun, Zhan-Min,Tang, Yi-Xiong,Wu, Yan-Min,Zhu, Xue-Mei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台