Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum
文献类型: 外文期刊
作者: Chen Shuai 1 ; Liu Guan-shan 1 ; Wang Yuan-ying 1 ; Sun Yu-he 1 ; Chen Jia 2 ;
作者机构: 1.Chinese Acad Agr Sci, Key Lab Genet Improvement & Biotechnol, Tobacco Res Inst, Qingdao 166101, Peoples R China
2.China Agr Univ, State Key Lab Plant Physiol & Biochem, Coll Biol Sci, Beijing 100193, Peoples R China
关键词: abiotic stress;CDPK;Nicotiana tabacum;RACE;real-time qRT-PCR
期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )
ISSN: 1671-2927
年卷期: 2011 年 10 卷 12 期
页码:
收录情况: SCI
摘要: Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca(2+) signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRT-PCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.
- 相关文献
作者其他论文 更多>>
-
Mycoplasma leachii causes bovine mastitis: Evidence from clinical symptoms, histopathology and immunohistochemistry
作者:Chang Ji-tao;Wang Fang;Jiang Zhi-gang;Yu Li;Yu De-bin;Liang Jian-bin;Chen Jia;Wang Jian-fa;Wu Rui;He Xi-jun
关键词:Mycoplasma leachii; bovine mastitis; pathogenicity; histopathology; immunohistochemistry
-
Isolation and expression analysis of NtCHS6, a new chalcone synthase gene from Nicotiana tabacum
作者:Chen Shuai;Cao Mo-ju;Pan Guang-tang;Chen Shuai;Zhang Yin-chao;Pan Xu-hao;Li Yi-ting;Cui Li-jie;Wu Feng-yan;Yang Ai-guo
关键词:chalcone synthase;Nicotiana tabacum;leaf-specific expression;light responsiveness;drought responsiveness
-
Cloning and Expression of Calcium-Dependent Protein Kinase (CDPK) Gene Family in Common Tobacco (Nicotiana tabacum)
作者:Tai Shuai-shuai;Liu Guan-shan;Sun Yu-he;Chen Jia
关键词:common tobacco (Nicotiana tabacum);CDPK;phylogenetic tree;gene expression



