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Effects of Pretreatment by the Flavanol Ampelopsin on Porcine Kidney Epithelial Cell Injury Induced by Hydrogen Peroxide

文献类型： 外文期刊

作者： Tan Gao-yi 1 ; Zhang Min-hong 1 ; Feng Jing-hai 1 ; Han Ai-yun 1 ; Zheng Shan-shan 1 ; Xie Peng 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

关键词： ampelopsin;PK-15 cell;antioxidation;hydrogen peroxide

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 4 期

页码：

收录情况： SCI

摘要： This study investigated the protective effects of ampelopsin on H(2)O(2)-induced oxidative injury in porcine kidney epithelial cell line, PK-15, cells were pretreated with medium containing 0, 15, 30, and 60 mu g mL(-1) ampelopsin, respectively, for 1 h prior to the addition of 100 mu mol L(-1) H(2)O(2) After 2 h, the cell viability, intracellular superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, cellular malondialdehyde (MDA) content, and DNA strand breakage were determined. Results showed that ampelopsin pretreatment did not modify the SOD and GSH-Px inactivation but significantly decreased MDA production, except for the medium dose (30 mu g mL(-1)), all the tested concentrations of ampelopsin did not significantly inhibit cell viability reduction and gave no influence on DNA damage The results suggested that all the tested doses of ampelopsin pretreatment before H(2)O(2) addition can protect PK-15 cell from H(2)O(2)-induced lipid peroxidation, which might not be mediated by the SOD and GSH-Px responses The appropriate dose of ampelopsin pretreatment prevents PK-15 cell from DNA damage and cell viability reduction

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