您好,欢迎访问中国农业科学院 机构知识库!

Preparation and Identification of a Monoclonal Antibody Specific to a Broad Spectrum of O, O-diethyl Organophosphorus Pesticides

文献类型: 外文期刊

作者: Zhang Li-Jie 1 ; Pan Jia-Rong 1 ;

作者机构: 1.Minist Agr, Inst Agrofood Sci & Technol, CAAS Key Lab Agofood Proc & Qual Control, Beijing 100193, Peoples R China

关键词: monoclonal antibody;broad spectrum;organophosphorous pesticides;ELISA

期刊名称:PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ( 影响因子:0.351; 五年影响因子:0.272 )

ISSN: 1000-3282

年卷期: 2010 年 37 卷 9 期

页码:

收录情况: SCI

摘要: The objective of this study was to develop an enzyme-linked immunosorbent assay (ELISA) with specific monoclonal antibodies for quick detection and screening of a broad spectrum of O,O-diethyl organophosphorus pesticides. Diethylphosphono acetic acid was used as a hapten and conjugated with bovine sera albumin (BSA) or ovalbumin (OVA) to prepare immunogen and coating antigen, respectively. Two hybridoma cell lines that produce monoclonal antibodies against organophosphorus pesticides have been obtained by fusing mouse myeloma cells SP2/0 and splenocytes from Balb/c mice immunized with the immunogen. The immunoglobulin class was analyzed by double immunodiffusion and the affinity and specificity of McAbs were determinated with indirect enzyme-linked immunosorbent assay (ELISA). Results indicated that the immunoglobulin class of McAb was IgG1, McAbs have a high affinity for diethylphosphono acetic acid (1.4x10(7) L/mol) and are able to cross-react to Chlorpyifos, Parathion, Profenofos, Omethoate, Dichlofenthion, Diazinon, Bromophos, Phoxim. This immunoassay is therefore capable of detecting 8 different organophosphorus pesticides. It is concluded that this assay is a qualified screening tool for quantitative or semi-quantitative detection of the 8 organophosphorous pesticides.

  • 相关文献

[1]Development of an indirect competitive ELISA for the detection of acenaphthene and pyrene. Qiao, Bin,Li, Yansong,Meng, Xingyu,Sun, Yu,Hu, Pan,Lu, Shiying,Ren, Honglin,Liu, Zengshan,Zhou, Yu,Meng, Xingyu,Sun, Yu,Zhou, Yu.

[2]Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins. Li, Peiwu,Zhou, Qian,Wang, Ting,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Zhou, Qian,Chang, Perng-Kuang. 2016

[3]A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for 5-Formyltetrahydrofolate Detection in Maize Kernels. Yue, Huanfang,Zhang, Wei,Cao, Zhen,Tan, Guiyu,Wang, Baomin,Yue, Huanfang,Liang, Qiuju,Zhang, Chunyi.

[4]Production and characterization of monoclonal antibody for class-specific determination of O,O-dimethyl organophosphorus pesticides and effect of heterologous coating antigens on immunoassay sensitivity. Liu, Yan,Lou, Yang,Xu, Dan,Qian, Guoliang,Wu, Runru,Hu, Baishi,Liu, Fengquan,Zhang, Qi.

[5]Analysis of eight organophosphorus pesticide residues in fresh vegetables retailed in agricultural product markets of Nanjing, China. Wang, Ligang,Jiang, Xin,Wang, Ligang,Liang, Yongchao,Wang, Ligang,Liang, Yongchao. 2008

[6]液相阻断ELISA检测口蹄疫病毒抗体的应用研究. 马军武. 2003

[7]口蹄疫病毒非结构蛋白-ELISA的研究和应用Ⅱ.NSP-ELISA鉴别FMD康复与免疫动物. 朱彩珠. 2003

[8]液相阻断ELISA检测口蹄疫病毒抗体方法的建立. 马军武. 2003

[9]不同致病型棉花黄萎病菌菌株产毒能力及致萎性比较. 西南农业大学,简桂良,许东,肖崇刚,中国农业科学院植物保护研究所. 2005

[10]猪流感抗体间接ELISA诊断方法的建立. 陈艳,辛晓光,杨焕良,李婷,李海燕,乔传玲,陈化兰. 2006

[11]磺胺对甲氧嘧啶抗血清的制备. 陈连颐,王捍东,王宗元. 2003

[12]番鸭呼肠孤病毒σB编码基因克隆、表达与间接ELISA诊断方法的建立. 郭东春,张云,耿宏伟,东北农业大学,胡奇林,郝雪,黑龙江八一农垦大学,欧阳岁东. 2006

[13]Development of Monoclonal Antibody-Based Sensitive Sandwich ELISA for the Detection of Antinutritional Factor Cowpea Trypsin Inhibitor. G. Y. Tan ,T. G. Nan,W. Gao,Q. X. Li,J. J. Cui,B. M. Wang.

[14]An ultra-sensitive monoclonal antibody-based competitive enzyme immunoassay for aflatoxin M-1 in milk and infant milk products. Guan, Di,Li, Peiwu,Zhang, Wen,Zhang, Daohong,Jiang, Jun,Guan, Di,Li, Peiwu,Zhang, Qi,Zhang, Wen,Zhang, Daohong,Jiang, Jun,Guan, Di,Li, Peiwu,Zhang, Qi. 2011

[15]Development of an antigen-capture ELISA for the detection of equine influenza virus nucleoprotein. Ji, Yuanyuan,Guo, Wei,Zhao, Liping,Li, Hongmei,Lu, Gang,Wang, Zheng,Wang, Guibin,Liu, Cuiyun,Xiang, Wenhua. 2011

[16]Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. Yun, Bingling,Li, Delong,Zhu, Haibo,Liu, Wen,Qin, Liting,Liu, Zaisi,Wu, Guan,Wang, Yongqiang,Qi, Xiaole,Gao, Honglei,Wang, Xiaomei,Gao, Yulong. 2013

[17]An approach for isolating high-molecular-weight glutenin subunit genes using monoclonal antibodies. Wang, HQ,Zhang, XY. 2006

[18]Identification of two antigenic epitopes on SARS-CoV spike protein. Hua, RH,Zhou, YJ,Wang, YF,Hua, YZ,Tong, GZ. 2004

[19]Development of a Monoclonal Antibody-Based Enzyme Immunoassay for the Pyrethroid Insecticide Deltamethrin. Kong, Ye,Zhang, Qi,Zhang, Wen,Li, Peiwu,Zhang, Wen,Li, Peiwu,Zhang, Qi,Gee, Shirley J..

[20]Detection of Tilletia controversa using immunofluorescent monoclonal antibodies. Liu, T.,Liu, B.,Chen, W.,Feng, C.,Li, B..

作者其他论文 更多>>