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Synthesis and Identification of Generic Antigen for Ether Pyrethroid Pesticides

文献类型： 外文期刊

作者： Sui Ke 1 ; Pan Jia-Rong 1 ; Wang Lei 1 ; Wang Zhi-Wei 1 ; Zhao Jie 1 ; Zhen Yue-Ming 1 ; Zhuge Jie-Jing 2 ; Pan Wen-Bin 2 ;

作者机构： 1.Chinese Acad Agr Sci, Agrofood Proc Inst, Beijing 100193, Peoples R China

2.China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China

3.Shenyang Agr Univ, Coll Food Sci, Shenyang 110866, Peoples R China

关键词： Ether pyrethroid pesticides;Generic antigen;Enzyme-linked immunosorbent assay

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2012 年 40 卷 8 期

页码：

收录情况： SCI

摘要： 2-(4-Ethoxypheny1)-2-methylpropanol can react with sodium chloroacetate by nuclephilic substitution reaction. Hapten I, as the product of the above reaction identified by H-1-NMR and C-13-NMR, was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) by the active ester method to get artificial immune antigen and coating antigen, respectively. The binding ratio of artificial antigens was detected by UV spectroscopy as 14:1 and 35:1. Balb/c mice were immunized with immunogen to prepare antiserum whose titer reached 1.28 X 10(5). The icELISA was conducted with the antiserum, and the IC50 was 0.2653 mg/L and the IC10 was 0.0012 mg/L.

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