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Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1

文献类型： 外文期刊

作者： Li GuangYue 1 ; Yang XiuFen 1 ; Zeng HongMei 1 ; Mao JianJun 1 ; Liu Hua 1 ; Zhang YunHua 1 ; Qiu DeWen 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Minist Agr, Key Lab Biol Control, Beijing 100081, Peoples R China

关键词： protein elicitor;stable isotope labelling;relative abundances;PeaT1

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

年卷期： 2010 年 53 卷 12 期

页码：

收录情况： SCI

摘要： The protein elicitor from the mycelium of Alternaria tenuissima has been isolated. The elicitor triggered resistance to the tobacco mosaic virus in tobacco by inducing relative oxygen species, but without causing hypersensitive necrosis. The elicitor is reported to impart resistance against Verticillium dahliae and to increase yield in cotton, but its mechanism is not yet clear. In this study, the stable isotope labelled mass spectrometry method was used to quantify the relative abundances of protein expression induced by PeaT1 in Arabidopsis. A significant difference in the relative abundances for the expression of different proteins related to metabolism, modification, regulatory, defense, stress and antioxidation was found in Arabidopsis.

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[15]Purification and expression of a protein elicitor from Alternaria tenuissima and elicitor-mediated defence responses in tobacco. Liu, Q.,Yang, X.,Long, C.,Zhao, M.,Zeng, H.,Liu, H.,Yuan, J.,Qiu, D..

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