您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system

文献类型： 外文期刊

作者： Khan, Sehroon 1 ; Nadir, Sadia 4 ; Guo Lihua 3 ; Xu, Jianchu 1 ; Holmes, Keith A. 6 ; Qiu Dewen 3 ;

作者机构： 1.World Agroforestry Ctr, East & Cent Asia, Kunming 650201, Yunnan, Peoples R China

2.Chinese Acad Sci, Ctr Mt Ecosyst Studies, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China

3.Chinese Acad Agr Sci, Minist Agr, Inst Plant Protect, Key Lab Integrated Pest Management Crops, 12 Zhong Guancun South St, Beijing 100081, Peoples R China

4.Yunnan Agr Univ, Rice Res Inst, Kunming 650201, Yunnan, Peoples R China

5.Univ Sci & Technol Bannu, Fac Sci, Dept Chem, Khyber Pakhtunkhwa 28100, Bannu, Pakistan

6.CABI, CH-2800 Delemont, Sw

关键词： Insect-toxic protein;Bb70p;Entomopathogenic fungi;Beauveria bassiana;Galleria mellonella;Filtrate

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： An insect-toxic protein, Bb70p, was purified from Beauveria bassiana 70 using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. Bb70p has a high affinity for anion exchangers and 2D electrophoresis results revealed a single spot with a molecular weight of 35.5 kDa and an iso-electric point of similar to 4.5. Bb70p remains active from 4 to 60 degrees C, within a pH range of 4-10, but is more active in slightly acidic pH. A pure protein, Bb70p does not have any carbohydrate side chains. The protein caused high mortality by intra-haemocelic injection into Galleria mellonella with LD50 of 334.41 mu g/g body weight and activates the phenol oxidase cascade. With a partial amino acid sequence comparison using the NCBI database, we showed no homology to known toxin proteins of entomopathogenic fungi. Thus, Bb70p appears to be an insect toxin protein, demonstrating novelty. Identification of this insect-toxic protein presents potential to enhance the virulence of B. bassiana through genetic manipulation. (C) 2015 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Entomopathogenicity of Three Muscardine Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae, against the Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). Ma, Chun-Sen,Majeed, M. Z.,Fiaz, M.,Afzal, M.. 2017

[2]Laboratory evaluation of virulence of Chinese Beauveria bassiana and Metarhizium anisopliae isolates to engorged female Rhipicephalus (Boophilus) microplus ticks. Ren, Qiaoyun,Liu, Zhijie,Guan, Guiquan,Sun, Ming,Ma, Miling,Niu, Qingli,Li, Youquan,Liu, Aihong,Liu, Junlong,Yang, Jifei,Yin, Hong,Luo, Jianxun. 2012

[3]Laboratory evaluation of Beauveria bassiana and Metarhizium anisopliae in the control of Haemaphysalis qinghaiensis in China. Ren, Qiaoyun,Chen, Ze,Luo, Jin,Liu, Guangyuan,Guan, Guiquan,Liu, Zhijie,Liu, Aihong,Li, Youquan,Niu, Qingli,Liu, Junlong,Yang, Jifei,Yin, Hong,Luo, Jianxun,Yin, Hong,Han, Xueqing.

[4]An insecticidal protein from Xenorhabdus ehlersii stimulates the innate immune response in Galleria mellonella. Zeng, HongMei,Yang, XiuFen,Liu, Zheng,Qiu, Dewen. 2013

[5]Characterization of surface antigen protein 1 (SurA1) from Acinetobacter baumannii and its role in virulence and fitness. Liu, Dong,Liu, Zeng-Shan,Hu, Pan,Li, Yan-Song,Lu, Shi-Ying,Liu, Nan-Nan,Ma, Xiao-Long,Chi, Dan,Chang, Jiang,Shui, Yi-Ming,Li, Zhao-Hui,Ahmad, Waqas,Zhou, Yu,Ren, Hong-Lin,Fu, Bao-Quan,Cai, Ling.

[6]An insecticidal protein from Xenorhabdus budapestensis that results in prophenoloxidase activation in the wax moth, Galleria mellonella. Zeng, Hong-Mei,Yang, Xiu-Fen,Liu, Zheng,Guo, Li-Hua,Yuan, Jing-Jing,Qiu, De-Wen,Yang, Jun,Lin, Hua-Feng,Yang, Jun,Zeng, Hong-Mei,Yang, Xiu-Fen,Liu, Zheng,Guo, Li-Hua,Yuan, Jing-Jing,Qiu, De-Wen. 2012

[7]Improvement of the propamocarb-tolerance of Lecanicillium lecanii through UV-light radiation-based mutagenesis. Xie, Ming,Li, Qian,Hu, Xin-Ping,Zhang, Yan-Jun,Peng, De-Liang,Zhang, Zhao-Rong,Li, Qiang,Liu, Zhi-Qi. 2018

[8]Further notes on the molecular taxonomy of Metarhizium. Huang, B,Humber, RA,Li, SG,Li, ZZ,Hodge, KT. 2005

[9]Susceptibility of the tick Haemaphysalis qinghaiensis to isolates of the fungus Metarhizium anisopliae in China. Ren, Qiaoyun,Sun, Ming,Guan, Guiquan,Liu, Zhijie,Chen, Ze,Liu, Aihong,Li, Youquan,Ma, Miling,Yang, Jifei,Niu, Qingli,Liu, Junlong,Yin, Hong,Luo, Jianxun,Han, Xueqing.

[10]Establishment of polyethylene-glycol-mediated protoplast transformation for Lecanicillium lecanii and development of virulence-enhanced strains against Aphis gossypii. Xie, Ming,Zhang, Xiao-Lin,Peng, De-Liang,Yu, Wen-Bin,Li, Qian,Zhao, Jin-Jin,Zhang, Zhao-Rong,Li, Qiang,Li, Qian.

[11]Potential of a strain of the entomopathogenic fungus Beauveria bassiana (Hypocreales: Cordycipitaceae) as a biological control agent against western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae). Gao, Yulin,Wang, Jing,Xu, Xuenong,Lei, Zhongren,Reitz, Stuart R.. 2012

[12]Inductive expression patterns of genes related to Toll signaling pathway in silkworm (Bombyx mori) upon Beauveria bassiana infection. Geng, Tao,Huang, Yuxia,Hou, Chengxiang,Qin, Guangxing,Lv, Dingding,Guo, Xijie,Hou, Chengxiang,Qin, Guangxing,Guo, Xijie. 2016

[13]Molecular cloning and characterization of High Mobility Group box (HMGB) gene from Beauveria bassiana- infected silkworm, Bombyx mori. Chengxiang, H.,Han, W.,Dingding, L.,Ruilin, L.,Xijie, G.,Chengxiang, H.,Han, W.,Dingding, L.,Ruilin, L.,Xijie, G.. 2017

[14]Laboratory and greenhouse evaluation of a new entomopathogenic strain of Beauveria bassiana for control of the onion thrips Thrips tabaci. Gao, Yulin,Xu, Xuenong,Zhang, Yaping,Wang, Jing,Lei, Zhongren,Smagghe, Guy.

[15]Bombyx mori cecropin A has a high antifungal activity to entomopathogenic fungus Beauveria bassiana. Lu, Dingding,Geng, Tao,Hou, Chengxiang,Huang, Yuxia,Qin, Guangxing,Guo, Xijie,Lu, Dingding,Geng, Tao,Hou, Chengxiang,Huang, Yuxia,Qin, Guangxing,Guo, Xijie,Lu, Dingding.

[16]Sublethal Effects of Beauveria bassiana (Ascomycota: Hypocreales) on Life Table Parameters of Frankliniella occidentalis (Thysanoptera: Thripidae). Wang, Haihong,Lei, Zhongren,Wang, Haihong.

[17]JAK/STAT signaling pathway-mediated immune response in silkworm (Bombyx mori) challenged by Beauveria bassiana. Geng, Tao,Lv, Ding-Ding,Huang, Yu-Xia,Hou, Cheng-Xiang,Qin, Guang-Xing,Guo, Xi-Jie,Hou, Cheng-Xiang,Qin, Guang-Xing,Guo, Xi-Jie.

[18]Effectiveness of Beauveria bassiana sensu lato strains for biological control against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in China. Sun, Ming,Ren, Qiaoyun,Guan, Guiquan,Yin, Hong,Luo, Jianxun,Sun, Ming,Li, Yufeng,Ma, Chao,Han, Xueqing.

[19]Compatibility of Beauveria bassiana with Neoseiulus barkeri for Control of Frankliniella occidentalis. Gao Yu-lin,Xu Xue-nong,Lei Zhong-ren,Goettel, Mark S.. 2015

[20]Bacillus thuringiensis Cry3Aa toxin increases the susceptibility of Crioceris quatuordecimpunctata to Beauveria bassiana infection. Liu, Chenxi,Lei, Zhongren,Oppert, Brenda,Lord, Jeffrey C.. 2012

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台