您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Comparative proteomic analyses of two Taxus species (Taxus X media and Taxus mairei) reveals variations in the metabolisms associated with paclitaxel and other metabolites

文献类型： 外文期刊

作者： Hao, Juan 1 ; Guo, Hong 1 ; Shi, Xinai 1 ; Wang, Ye 1 ; Wan, Qinghua 1 ; Song, Yao-Bin 1 ; Zhang, Lei 1 ; Dong, Ming 1 ; Shen, Chenjia 2 ;

作者机构： 1.College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China;Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310036, China

2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China;^Hangzhou Normal Univ, Zhejiang Prov Key Lab Genet Improvement & Qual Co, Hangzhou 310036, Zhejiang, Peoples R China

3.College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China

4.Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province 455000, China

5.College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China;Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China

6.Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA

关键词： flavonoid;LC-MS/MS;proteomic analyses;Taxus;Taxol;TMT labeling

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

ISSN： 0032-0781

年卷期： 2017 年 58 卷 11 期

页码：

收录情况： SCI

摘要： Taxus species are well-known for paclitaxel, which exhibits antitumor activities and is used for treating various cancers. Although most Taxus species are widespread in many areas, few studies have characterized the variation in metabolism among different Taxus species. Using an integrated approach involving 'tandem mass tag' labeling and liquid chromatography-tandem mass spectrometry (HPLC-MS), proteomes of T. media and T. mairei were investigated and 4078 proteins were quantified. Screening and classification of differentially expressed proteins revealed many metabolism-associated proteins. In detail, four enzymes involved in the flavonoid biosynthesis pathway were predominantly expressed in T. mairei. Four enzymes associated with supplying precursors for paclitaxel biosynthesis and three cytochrome P450 taxoid oxygenases were preferentially expressed in T. media compared with T. mairei. Furthermore, variations in taxoid contents between T. media and T. mairei were determined using HPLC-MS analysis. Variations in flavonoid contents between T. media and T. mairei were determined by HPLC analysis. A number of differentially expressed proteins may provide an explanation for the variation in metabolisms of different Taxus species.

  • 相关文献

[1]A protocol of homozygous haploid callus induction from endosperm of Taxus chinensis Rehd. var. mairei. Li, Yan-Lin,Huang, San-Wen,Xiong, Xing-Yao,Li, Yan-Lin,Huang, San-Wen,Bu, Feng-Jiao,Lin, Tao,Zhang, Zhong-Hua,Xiong, Xing-Yao,Zhang, Jia-Yin. 2016

[2]Sequence-related amplified polymorphism (SRAP) marker as a new method for identification of endophytic fungi from Taxus. Ren, Na,Liu, Jiajia,Yang, Dongliang,Hong, Juan,Chen, Jianhua,Luan, Mingbao. 2012

[3]晋麦47幼苗中一个水分胁迫应答蛋白的SDS-PAGE和Nano LC-MS/MS鉴定. 范锋贵,张晓科,任万杰,王晓龙,叶石,付晓洁,王宏礼,朱建楚. 2012

[4]农产品与饲料中T-2毒素免疫亲和柱净化-液相色谱串联质谱检测技术研究. 李静,马飞,李培武,张奇,丁小霞,张文. 2014

[5]黄瓜根分泌物中的7;8-苯并黄酮及其对枯萎病发生的影响. 李世东,郭荣君. 2010

[6]玉米DDGS中抗生素残留分析. 姚婷,秦玉昌,吴宁鹏,彭丽,孙丹丹,谷旭. 2014

[7]利用多功能针式过滤器快速检测油菜和苹果中68种农药及代谢物残留. 贾琪,王纯强,马蕊,王宇彤,钱永忠,邱静. 2019

[8]荷斯坦奶牛瘤胃微生物脲酶的分离与鉴定. 赵圣国,王加启,刘开朗,李旦,于萍,卜登攀. 2010

[9]LC-MS/MS测定青稞中氨基酸的含量及主成分分析. 王猛强,邱城,张唐伟,李腾飞,李壮,王淼,曹振,王珊珊,郑鹭飞,金茂俊,王静,邵华,金芬,佘永新. 2022

[10]黄瓜根分泌物中的7,8-苯并黄酮及其对枯萎病发生的影响. 李世东,郭荣君. 2010

[11]液相色谱-串联质谱法检测鸡蛋中金刚烷胺残留. 汤晓艳,陈东宇,周剑,毛雪飞,邱静,王敏. 2014

[12]阿维菌素在柑桔和土壤中的残留及其消解动态. 张娇娇,刘炎,叶玉凤,赵其阳,彭震宇,焦必宁,王成秋. 2017

[13]基于分散固相萃取液相色谱-串联质谱法测定大米中8种真菌毒素. 孙伟华,杨欢,曹赵云,马有宁,秦美玲,柴爽爽,陈铭学. 2017

[14]大丽轮枝菌分泌蛋白提取方法比较. 肖红利,桂月晶,祁伟彦,陈捷胤,李蕾,徐明,戴小枫. 2014

[15]LC-MS/MS检测饲料中二甲氧苄啶、甲氧苄啶和二甲氧甲基苄啶方法的建立. 杨亚军,刘希望,李冰,孔晓军,李世宏,秦哲,杜文斌,李剑勇. 2015

[16]蔬菜中啶虫脒残留免疫分析方法研究. 刘蓓蓓,吕军,张杰,刘广洋,翟荣启,陈鸽,徐东辉. 2025

[17]Nutritional composition and antioxidant activity of twenty mung bean cultivars in China. Shi, Zhenxing,Yao, Yang,Zhu, Yingying,Ren, Guixing,Zhu, Yingying. 2016

[18]A mutation in the rice chalcone isomerase gene causes the golden hull and internode 1 phenotype. Hong, Lilan,Tang, Ding,Wang, Kejian,Li, Ming,Cheng, Zhukuan,Qian, Qian.

[19]Characterization of phenolic profile and antioxidant capacity of different fruit part from lemon (Citrus limon Burm.) cultivars. Xi, Wanpeng,Lu, Juanfang,Qun, Junping,Jiao, Bining,Jiao, Bining.

[20]Quality evaluation of snow lotus (Saussurea): quantitative chemical analysis and antioxidant activity assessment. Qiu, Jian,Chen, Fudong,Zhao, Dexiu,Qiu, Jian,Chen, Fudong,Li, Chonghui,Zhao, Qiao,Xue, Xiaofeng,Bolat, Nuer,Wang, Xiaojun,Baima, Yuzhen,Ma, Fengshan.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台