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Allelic Variation at the TaZds-A1 Locus on Wheat Chromosome 2A and Development of a Functional Marker in Common Wheat

文献类型: 外文期刊

作者: Dong Chang-hai 1 ; Ma Zhi-ying 1 ; Xia Xian-chun 2 ; Zhang Li-ping 2 ; He Zhong-hu 2 ;

作者机构: 1.Agr Univ Hebei, Coll Agron, Baoding 071001, Peoples R China

2.CAAS, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

3.CAAS, CIMMYT China Off, Beijing 100081, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China

关键词: Triticum aestivum;functional marker;QTL;zeta-carotene desaturase;ZDS

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2012 年 11 卷 7 期

页码:

收录情况: SCI

摘要: zeta (zeta)-carotene desaturase (ZDS) is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment (YP) content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame (ORF) of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-A1a and TaZds-A1b, yielding 183- and 179-bp fragments in TaZds-A1 a and TaZds-A1b genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid (DH) population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-A1b allele was 7.8% higher than that of 91 cultivars with TaZds-A1a allele.

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