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Characterization of TaCOMT genes associated with stem lignin content in common wheat and development of a gene-specific marker

文献类型： 外文期刊

作者： Fu Lu-ping 1 ; Xiao Yong-gu 1 ; Yan Jun 1 ; Liu Jin-dong 2 ; Wen Wei-e 1 ; Zhang Yong 1 ; Xia Xian-chun 1 ; He Zhong-hu 3 ;

作者机构： 1.National Wheat Improvement Center/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

2.Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China

3.National Wheat Improvement Center/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;International Maize and Wheat Improvement Center (CIMMYT) China Office, Beijing 100081, P.R.China

关键词： Triticum aestivum L.; caffeic acid 3-O-methyltransferase; stem lignin content; gene-specific marker; lodging resistance

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2019 年 18 卷 5 期

页码：

收录情况： SCI

摘要： Stem lignin content (SLC) in common wheat (Triticum aestivum L.) contributes to lodging resistance. Caffeic acid 3-O-methyltransferase (COMT) is a key enzyme involved in lignin biosynthesis. Characterization of TaCOMT genes and development of gene-specific markers could enable marker-assisted selection in wheat breeding. In the present study, the full-length genomic DNA (gDNA) sequences of TaCOMT genes located on chromosomes 3A, 3B, and 3D were cloned by homologous cloning. Two allelic variants, TaCOMT-3Ba and TaCOMT-3Bb, were identified and differed by a 222-bp insertion/deletion (InDel) in the 3'-untranslated region (3'-UTR). A co-dominant gene-specific marker based on this InDel was developed and designated as TaCOMT-3BM. A total of 157 wheat cultivars and advanced lines grown in four environments were used to validate the associations between allelic patterns and SLC. The SLC of cultivars with TaCOMT-3Ba was significantly (P<0.01) higher than that of those with TaCOMT-3Bb, and the marker TaCOMT-3BM could be effectively used in wheat breeding.

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