您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Inhibitory effect and mechanism of mesenchymal stem cells on liver cancer cells

文献类型： 外文期刊

作者： Hou, Lingling 1 ; Wang, Xiaoyu 1 ; Zhou, Yaqiong 1 ; Ma, Haibin 1 ; Wang, Ziling 1 ; He, Jinsheng 1 ; Hu, Honggang 1 ; Gu 1 ;

作者机构： 1.Beijing Jiaotong Univ, Coll Life Sci & Bioengn, Beijing 100044, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

关键词： Mesenchymal stem cells;Liver cancer cells;Inhibition;Mechanism

期刊名称：TUMOR BIOLOGY （ 影响因子：3.65； 五年影响因子：3.445 ）

ISSN： 1010-4283

年卷期： 2014 年 35 卷 2 期

页码：

收录情况： SCI

摘要： Mesenchymal stem cells (MSCs), with their capacity for self-renewal and differentiation into various cell types, are important seed cells for stem cell therapy. MSCs exhibit potent pathotropic migratory properties that make them attractive for use in tumor prevention and therapy. However, little is known about the underlying molecular mechanisms that link MSCs to the targeted tumor cells. This study investigated the inhibitory effect and mechanism of MSCs on human hepatoma HepG(2) cells using co-culture and conditioned medium system and animal transplantation model. The HepG(2) cells were co-cultured with MSCs or treated with conditional media derived from MSCs cultures in vitro. Results of methylthiazolyldiphenyl tetrazolium assay and flow cytometric assay showed that the proliferation and apoptosis of HepG(2) cells decreased and increased, respectively. Reverse transcription polymerase chain reaction analysis showed that the expression levels of bcl-2, c-Myc, beta-catenin, and survivin were downregulated. The results of enzyme-linked immunosorbent assay and Western blot proved that MSCs secreted Dkk-1 to inhibit the expression of Wnt signaling pathway-related factors (bcl-2, c-Myc, beta-catenin, and survivin) in tumor cells, consequently inhibiting the proliferation and promoting the apoptosis of HepG(2) cells. Animal transplantation experiment showed that tumor growth was significantly inhibited when HepG(2) cells were co-injected with MSCs into nude mice. These results suggested that MSCs inhibited the growth and promoted the apoptosis of HepG(2) cells in a dose-dependent manner. This study provided a new approach and experimental basis for cancer therapy. This study also proved that the Wnt signaling pathway may have a function in MSC-mediated tumor cell inhibition.

  • 相关文献

[1]The Inhibitory Effect of Mesenchymal Stem Cells with rAd-NK4 on Liver Cancer. Cai, Chao,Hou, Lingling,Zhang, Jingsi,Zhao, Diandian,Wang, Ziling,Hu, Honggang,He, Jinsheng,Guan, Weijun,Ma, Yuehui.

[2]Phosphomannose-isomerase (pmi) gene as a selectable marker for rice transformation via Agrobacterium. He, ZQ,Fu, YP,Si, HM,Hu, GC,Zhang, SH,Yu, YH,Sun, ZX.

[3]黄金梨黑心病形成机理及防控技术研究. 王文辉,王志华,佟伟,张志云,姜修成,贾晓辉. 2008

[4]ESTIMATING THE PHOTOSYNTHETIC INHIBITION BY ULTRA VIOLET RADIATION ON THE ANTARCTIC PHYTOPLANKTON ALGAE*. CARLOS MARIO OCAMPO MARTINEZ,FERNANDO ROBERTO MOMO,GLADYS ELENA SALCEDO ECHEVERRY. 2011

[5]MicroRNAs can effectively induce formation of insulin-producing cells from mesenchymal stem cells. Bai, Chunyu,Gao, Yuhua,Li, Xiangchen,Wang, Kunfu,Xiong, Hui,Shan, Zhiqiang,Zhang, Ping,Wang, Wenjie,Guan, Weijun,Ma, Yuehui. 2017

[6]Isolation and characterization of mesenchymal stem cells from chicken bone marrow. Bai, Chunyu,Ma, Yuehui,Chen, Lina,Guan, Weijun,Bai, Chunyu,Zhang, Minghai,Hou, Lingling. 2013

[7]Staphylococcus aureus infection of intestinal epithelial cells induces human umbilical cord-derived mesenchymal stem cell migration. Li, Yang,Liu, Ming-yuan,Wang, Xue-lin,Han, Wen-yu,Liu, Ya-hui,Suo, Jian,Li, Zhi-jie,Li, Ya-gang,Jin, Hu. 2013

[8]Enhanced Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Graphene Oxide-Incorporated Electrospun Poly(lactic-co-glycolic acid) Nanofibrous Mats. Luo, Yu,Shen, He,Cao, Yuhua,Huang, Jie,Zhang, Mengxin,Dai, Jianwu,Zhang, Zhijun,Luo, Yu,Shi, Xiangyang,Fang, Yongxiang,Dai, Jianwu,Fang, Yongxiang. 2015

[9]MicroRNA-34c acts as a bidirectional switch in the maturation of insulin-producing cells derived from mesenchymal stem cells. Bai, Chunyu,Yang, Wancai,Gao, Yuhua,Zhang, Xiangyang,Bai, Chunyu,Gao, Yuhua,Guan, Weijun,Yang, Wancai. 2017

[10]Wip1 knockout inhibits the proliferation and enhances the migration of bone marrow mesenchymal stem cells. Tang, Yiting,Sheng, Ming,Liu, Honglin,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Xiong, Kai. 2015

[11]Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells. Bai, Chunyu,Gao, Yuhua,Li, Qian,Feng, Yuan,Yu, Yanze,Meng, Gentong,Zhang, Minghai,Bai, Chunyu,Gao, Yuhua,Li, Qian,Guan, Weijun.

[12]Biological characterization of mesenchymal stem cells from bovine umbilical cord. Xiong, Hui,Bai, Chunyu,Wu, Shuang,Gao, Yuhua,Lu, Taofeng,Guan, Weijun,Ma, Yuehui,Xiong, Hui,Hu, Qingyun.

[13]Differentiation potential of bone marrow mesenchymal stem cells in duck. Li, Linfeng,Gong, Xuelian,Liu, Hongkun,Chen, Lina,Guan, Weijun,Ma, Yuehui,Li, Linfeng,Bai, Xiujuan. 2009

[14]BMP2 Immobilisation on Extracellular Matrices to Generate an Interactive Niche for Human Mesenchymal Stem Cells. F. Philipp Seib,Milauscha Grimmer,Tina Lenk,Katrin Salchert,Katrin Mueller,Martin Bornhaeuser,Carsten Werner. 2007

[15]TiO2 Nanowired Cerebrolysin enhances neuroprotective effects of mesenchymal stem cells following concussive head injury at hot environments. Hari S. Sharma,Dafin F. Muresanu,Lianyuan Feng,Jose Vicente Lafuente,Ranjana Patnaik,Z. Ryan Tian,Asya Ozikzilcik,Herbert Mossler,Aruna Sharma. 2015

[16]Chapter 20. Size-Exclusion Effect and Protein Repellency of Concentrated Polymer Brushes Prepared by Surface-Initiated Living Radical Polymerization. Chiaki Yoshikawa,Atsushi Goto,Norio Ishizuka,Kazuki Nakanishi,Akio Kishida,Yoshinobu Tsujii,Takeshi Fukuda. 2007

[17]Paying for Watershed Services: Mechanisms, Modes and Compensation Methods in the River Basin, China Case Study of Jinhua River. ZHENG Haixia,XU Dawei,ZHANG Lubiao. 2010

[18]Application of Methane Fermentative Residues in the Control of Crop Diseases and Insect Pests. Zhang Wudi,Yin Fang,Li Jianchang,Xu Rui,Chen Yubao,Xu Ling,Mao Yu. 2008

[19]Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection. Ishag, Hassan Z. A.,Li, Chen,Wang, Fengjuan,Mao, Xiang,Mao, Xiang,Ishag, Hassan Z. A..

[20]Inhibition and recovery of biomarkers of earthworm Eisenia fetida after exposure to thiacloprid. Zhang, Lan,Zhang, Yanning,Zhang, Pei,Jiang, Hongyun.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台