您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Detection and sequence analysis of grapevine virus B isolates from China

文献类型： 外文期刊

作者： Hu, G. J. 1 ; Dong, Y. F. 1 ; Zhang, Z. P. 1 ; Fan, X. D. 1 ; Fang, R. 1 ; Zhu, H. J. 1 ;

作者机构： 1.Chinese Acad Agr Sci, Res Inst Pomol, Natl Ctr Eliminating Viruses Deciduous Fruit Tree, Xingcheng 125100, Liaoning, Peoples R China

关键词： GVB;detection;sequence alignment;phylogeny

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN： 0001-723X

年卷期： 2014 年 58 卷 2 期

页码：

收录情况： SCI

摘要： The presence of grapevine virus B (GVB) was detected in 188 grapevine samples from China by double antibody sandwich ELISA (DAS-ELISA) and reverse transcription-PCR (RT-PCR). The accuracy of detection by RT-PCR was confirmed by sequencing amplified PCR fragments. Seventeen samples were GVB-positive by DAS-ELISA and five by RT-PCR. The isolate QMW proved to be positive by RT-PCR only, and four isolates (DGWH, DGW, QM, and JFL) could be detected by both methods. Among the five GVB-positive samples detected by RT-PCR, two isolates were originally collected from Henan province and three from Liaoning province. The expected 722 bp DNA fragment, covering partial ORF3 through partial ORF5, was amplified from the five GVB infected samples. Sequence analysis revealed that the molecular variants' composition of GVB in the different isolates was complex. Clones of DGWH, DGW, QM, and JFL isolate shared high nucleotide identities, while the identities among the clones of isolate QMW varied. The variants of GVB isolates obtained in this study showed nucleotide identities from 81.1% to 97.9% among themselves, and 79.1% to 98.5% identity with five previously published GVB isolates in NCBI. The alignment of partial ORF3 and the phylogenetic relationships of ORF4 revealed that the molecular variants of Chinese GVB isolates could be clustered into three groups. Only isolate DGW was in the same group with the reported GVB isolates from other countries; the other four GVB isolates in this study were clustered into two groups.

  • 相关文献

[1]Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.). Li, Ai-Li,Zhu, Yuan-Fang,Tan, Xiao-Mei,Wang, Xiang,Wei, Bo,Guo, Han-Zi,Zhang, Zeng-Lin,Chen, Xiao-Bo,Zhao, Guang-Yao,Kong, Xiu-Ying,Jia, Ji-Zeng,Mao, Long,Tan, Xiao-Mei.

[2]A novel simple extracellular leucine-rich repeat (eLRR) domain protein from rice (OsLRR1) enters the endosomal pathway and interacts with the hypersensitive-induced reaction protein 1 (OsHIR1). Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhang, Qi,Lei, Cai-Lin,Zhang, Shi-Hong.

[3]Genome-wide analysis of SINA family in plants and their phylogenetic relationships. Wang, Meng,Jin, Ying,Fu, Junjie,Zhu, Yun,Hu, Jian,Wang, Guoying,Zheng, Jun,Wang, Guoying.

[4]Genome-wide analysis of AP2/ERF transcription factor family in Zea mays. Zhou, Mei-Liang,Tang, Yi-Xiong,Wu, Yan-Min,Zhou, Mei-Liang.

[5]Goose haemorrhagic hepatitis caused by a new subtype duck hepatitis type 1 virus. Liu, Ming,Fanyi-Meng,Li, Xiao-jun,Zhang, Zhuo,Liu, Siguo,Zhang, Yun.

[6]Molecular characterization and phylogenetic analysis of transmissible gastroenteritis virus HX strain isolated from China. Hu, Xiaoliang, Jr.,Li, Nannan, Jr.,Qu, Juanjuan,Hu, Xiaoliang, Jr.,Yin, Xin, Jr.,Tian, Zhige, Jr.,Qu, Liandong. 2015

[7]A Walking Assistive Device with Intention Detection using Back-driven Pneumatic Artificial Muscles. Takahiro Kanno,Daisuke Morisaki,Ryoken Miyazaki,Gen Endo,Kenji Kawashima. 2015

[8]Detection of Lymph Nodes in T2 MRI Using Neural Network Ensembles. Zhiyong Lu,Ronald M. Summers,Tejas Sudharshan Mathai,Sungwon Lee,Daniel C. Elton,Thomas C. Shen,Yifan Peng. 2021

[9]Universal Lesion Detection and Classification Using Limited Data and Weakly-Supervised Self-training. Varun Naga,Tejas Sudharshan Mathai,Angshuman Paul,Ronald M. Summers. 2022

[10]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[11]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[12]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[13]Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification. Liu, Yong-sheng,Liu, Xiang-tao. 2011

[14]The Applications of Gold Nanoparticle-Initialed Chemiluminescence in Biomedical Detection. Liu, Zezhong,Zhao, Furong,Gao, Shandian,Shao, Junjun,Chang, Huiyun. 2016

[15]A Miniature Handheld Automatic Detected Device for Register. Zhang Tiefeng,Wang Jun,Sun Zhihui,Zhao Bo,Lu Guotao. 2011

[16]DEVELOPMENT OF A SIMPLE AND EFFECTIVE METHOD FOR SPECIFIC DETECTION OF PEPPER MILD MOTTLE VIRUS. Liu, Y.,Wang, X.,Zhou, G.,Li, X.. 2009

[17]Surface-enhanced Raman spectroscopy (SERS) combined techniques for high-performance detection and characterization. Zhang, Ye,Zhao, Shaojie,Zheng, Jinkai,Zheng, Jinkai,He, Lili.

[18]Detection of Tilletia controversa using immunofluorescent monoclonal antibodies. Liu, T.,Liu, B.,Chen, W.,Feng, C.,Li, B..

[19]Development and application of a universal and simplified multiplex RT-PCR assay to detect five potato viruses. Wen, Jingzhi,Zhang, Wei,Fan, Guoquan,Gao, Yanling,Bai, Yanju,Qiu, Cailing,Zhang, Shu,Shen, Yu,Zhang, Zhixiang,Meng, Xianxin.

[20]Detection and differentiation of influenza viruses with glycan-functionalized gold nanoparticles. Zheng, Longtang,Lv, Xun,Bi, Yuhai,Zhang, Zhenxing,Wang, Pengfei,Liu, Ruichen,Jiang, Jingwen,Cong, Haolong,Liu, Wenjun,Gao, George F.,Li, Xuebing,Li, Xuebing,Zheng, Longtang,Zhang, Zhenxing,Wang, Pengfei,Jiang, Jingwen,Li, Xuebing,Wei, Jinhua,Du, Yuguang,Wu, Peixing,Liang, Jingnan,Cheng, Wenwen,Jiang, Xingyu,Cao, Hongzhi,Bi, Yuhai,Liu, Wenjun,Gao, George F.,Li, Xuebing,Cheng, Wenwen.

作者其他论文 更多>>
  • First Report of Grapevine Asteroid Mosaic-Associated Virus in Grapevines in China

    作者：Fan, X. D.;Dong, Y. F.;Zhang, Z. P.;Ren, F.;Hu, G. J.

    关键词：fruit; pathogen detection; viruses and viroids

  • EVALUATION OF THE ELIMINATION EFFICENCY OF APPLE STEM GROOVING VIRUS BY DIFFERENT DETECTION METHODS

    作者：Hu, G. J.;Dong, Y. F.;Zhang, Z. P.;Fan, X. D.;Ren, F.

    关键词：Apple stem grooving virus; Detection efficiency; RT-duplex PCR; Sensitivity; Various apple plants; Virus detection

  • Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation

    作者：Tzanetakis, I. E.;Atanda, H. Y.;Osundahunsi, B.;Sierra-Mejia, A.;Singh, S.;Stainton, D.;Villamor, D. E.;Aknadibossian, V.;Folimonova, S. Y.;Spak, J.;Franova, J.;Koloniuk, I.;Constable, F.;Harper, S. J.;Hammond, J.;Jordan, R.;Candresse, T.;Marais, A.;Freitas-Astua, J.;Barbosa, C.;Girardi, E.;Laranjeira, F.;Stuchi, E.;Fuchs, M.;Jelkmann, W.;Zikeli, K.;Maliogka, V. I.;Katis, N.;Martin, R. R.;Mollov, D.;Wang, J.;Vidalakis, G.;Lavagi-Craddock, I.;Mitra, A.;Aboughanem-Sabanadzovic, N.;Al Rwahnih, M.;Alabi, O. J.;Alioto, D.;Minutolo, M.;Bagi, F.;Baranwal, V. K.;Sharma, S. K.;Bar-Joseph, M.;Varma, A.;Batista Le Riverend, L.;Belien, T.;Benitez-Galeano, M. J.;Bennypaul, H.;Gaafar, Y. Z. A.;Gratz, A.;Bertaccini, A.;Bester, R.;Maree, H. J.;Blouin, A. G.;Blystad, D. -R.;Hamborg, Z.;Botermans, M.;Roenhorst, A.;Schoen, R.;Westenberg, M.;Bozan, O.;Onelge, N.;Brakta, A.;Brans, Y.;Bulajic, A.;Caglayan, K.;Catara, A.;Choueiri, E.;Cieslinska, M.;Cook, G.;Cui, W.;Fiore, N.;da Graca, J.;Davino, S.;Panno, S.;Delmiglio, C.;Perez-Egusquiza, Z.;Tang, J.;Thompson, J.;Veerakone, S.;Dewdney, M. M.;Levy, A.;Shilts, T.;Dewdney, M. M.;Levy, A.;Shilts, T.;Di Serio, F.;Minafra, A.;Navarro, B.;Saldarelli, P.;Diaz-Lara, A.;Digiaro, M.;Djelouah, K.;Dong, Y. F.;Donovan, N.;Wulff, N. A.;Donovan, N.;Druciarek, T. Z.;Duran-Vila, N.;Moreno, P.;Navarro, L.;Olmos, A.;Ruiz-Garcia, A. B.;Vives, M. C.;Elci, E.;Ulubas Serce, C.;Esquivel-Farina, A.;Fall, M. L.;Fall, M. L.;Fan, X. D.;Figueroa, J.;Palacios, M. F.;Fowkes, A. R.;Fox, A.;Skelton, A.;Vazquez-Iglesias, I.;Fuchs, R.;Garcia, M. L.;Ghosh, D.;Glasa, M.;Gomez Talquenca, S.;Gritsenko, D.;Hajeri, S.;Hajizadeh, M.;Ho, T.;Holeva, M.;Varveri, C.;Holkar, S. K.;Horner, M.;Hurtado-Gonzales, O. P.;Ippolito, A.;Isac, V.;Iwanami, T.;Jofre-y-Garfias, A. E.;Konings, H.;Meekes, E. T. M.;Krizanac, I.;Krueger, R.;Kyrychenko, A.;Licciardello, G.;Lu, Q. -Y.;MacFarlane, S. A.;Marcone, C.;Margaria, P.;Menzel, W.;Winter, S.;Martic, A.;Massart, S.;Tahzima, R.;Mathioudakis, M. M.;Matic, S.;Turina, M.;Mavric Plesko, I.;Mehle, N.;Melzer, M. J.;Meng, B.;Miljanic, V.;Stajner, N.;Nerva, L.;Okic, A.;Pallas, V.;Poudel-Ward, B.;Wright, G.;Radisek, S.;Ramos-Gonzalez, P. L.;Ramteke, P.;Ranabhat, N. B.;Rivarez, M. P. S.;Rivas, F.;Roy, A.;Sabanadzovic, S.;Safarova, D.;Salem, N.;Sanahuja Solsona, G.;Umble, J.;Skoric, D.;Starovic, M.;Svoboda, P.;Tessitori, M.;Thermoz, J. -P.;Thompson, E.;Trebicki, P.;Valiunas, D.;Varallyay, E.;Vasquez-Gutierrez, U.;Voncina, D.;Wetzel, T.;Xu, W. X.;Yokomi, R.;Zhou, C.;Zindovic, J.

    关键词：fruit; ornamentals; small fruits; tree fruits; viruses and viroids

  • Elimination of Apple stem pitting virus from in vitro-cultured pear by an antiviral agent combined with thermotherapy

    作者：Hu, G. J.;Hong, N.;Wang, G. P.;Hu, G. J.

    关键词：Pear; In vitro culture; Apple stem pitting virus (ASPV); Chemotherapy; Thermotherapy; Virus elimination

  • COMPLETE NUCLEOTIDE SEQUENCE OF A NEW ISOLATE OF GRAPEVINE VIRUS B FROM CHINA

    作者：Hu, G. J.;Dong, Y. F.;Zhang, Z. P.;Fan, X. D.;Ren, F.;Zhu, H. J.

    关键词：Vitivirus;Grapevine virus B;genome;sequence analysis

  • Lycopene content and expression of phytoene synthase and lycopene beta-cyclase genes in tetraploid watermelon

    作者：Yuan, P. L.;Liu, W. G.;Zhao, S. J.;Lu, X. Q.;Yan, Z. H.;He, N.;Zhu, H. J.

    关键词：Tetraploid watermelon;different tissues;fruit development;gene expression;lycopene accumulation

  • A novel double T-DNA system for producing stack and marker-free transgenic plants

    作者：Wang, X. J.;Su, Y. Y.;Dong, Y. F.;Tang, Q. L.;Wang, Z. X.

    关键词：Agrobacterium tumefaciens;isocaudamer technique;multi-gene stack;Nicotiana tabacum

置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台