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Elimination of Apple stem pitting virus from in vitro-cultured pear by an antiviral agent combined with thermotherapy

文献类型： 外文期刊

作者： Hu, G. J. 1 ; Hong, N. 1 ; Wang, G. P. 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Key Lab Agromicrobiol, Wuhan 430070, Hubei, Peoples R China

2.Chinese Acad Agr Sci, Res Inst Pomol, Natl Ctr Eliminating Viruses Deciduous Fruit Tree, Xingcheng 125100, Liaoning, Peoples R China

关键词： Pear; In vitro culture; Apple stem pitting virus (ASPV); Chemotherapy; Thermotherapy; Virus elimination

期刊名称：AUSTRALASIAN PLANT PATHOLOGY （ 影响因子：1.599； 五年影响因子：1.708 ）

ISSN： 0815-3191

年卷期： 2019 年 48 卷 2 期

页码：

收录情况： SCI

摘要： Six in vitro pear cultivars, Wonhwang, Xuehua, Conference, Stankimson, Starcrimson and Red Bastlett were infected by Apple stem pitting virus (ASPV) and were treated by combination of 25g/ml ribavirin and temperature 35 degrees C. Results showed that ribavirin could enhance the proliferation of Xuehua, Conference, Stankimson and Starcrimson and death plants were found in Xuehua and Red Bastlett and the total survival rate of the six cultivars was 93.5%. After treatment, the regenerated plants were detected by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that ASPV in all regenerated plants could not be detected and the total elimination rate of the six cultivars was 92.3%. The combination showed high efficacy on elimination of ASPV.

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