Determination of Alkaloids in Honey by Dispersive Solid Phase Extraction and High Performance Liquid Chromatography-Tandem Mass Spectrometry
文献类型: 外文期刊
作者: Guo Wei-Hua 1 ; Zhou Jin-Hui 1 ; Huang Jing-Ping 1 ; Wang Peng 1 ; Li Yi 1 ;
作者机构: 1.Chinese Acad Agr Sci, Risk Assessment Lab Bee Prod Qual & Safety, Qual Supervis & Testing Ctr Bee Prod, Bee Res Inst,Minist Agr, Beijing 100093, Peoples R China
关键词: Dispersive solid phase extraction;High performance liquid chromatography-tandem mass spectrometry;Pyrrolizidine alkaloids;Isoquinoline alkaloids;Honey
期刊名称:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.134; 五年影响因子:0.909 )
ISSN: 0253-3820
年卷期: 2014 年 42 卷 10 期
页码:
收录情况: SCI
摘要: A method was developed for simultaneous determination of pyrrolizidine alkaloids and isoquinoline alkaloids in honey by dispersive solid phase extraction and high performance liquid chromatography. tandem mass spectrometry (QuEChERS-HPLC-MS/MS). The honey samples were extracted with acetonitrile solution and cleaned up with PSA absorbent. Agilent Poroshell 120 SB-C-18 chromatographic column was used to separate alkaloids with high sensitivity and satisfactory resolution. The identification and quantification were achieved by using electrospray ionization in positive ion mode (ESI+) with multiple reaction monitoring (MRM). Matrix. matched calibration curves with good correlation coefficients (R-2 > 0.99) were obtained in the concentration range of 0.1-100 mu g/L. The recoveries of the spiked samples at 1-100 mu g/kg were in the range of 70% to 110% with the RSD of intra. day and inter. day lower than 15% and 20%, respectively. The limits of detection (LOD) and limits of quantification (LOQ) for all alkaloids were 0.3 and 1.0 mu g/kg, respectively. This method was successfully applied to the simultaneous determination of pyrrolizidine alkaloids and isoquinoline alkaloids for quantification and confirmation in honey samples.
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