Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library
文献类型: 外文期刊
作者: Gao, Y. H. 1 ; Wang, K. F. 1 ; Zhang, S. 1 ; Fan, Y. N. 1 ; Guan, W. J. 1 ; Ma, Y. H. 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
2.Northeast Forestry Univ, Coll Wildlife Resources, Harbin, Peoples R China
关键词: cDNA library;Hepatocytes;Liver fatty acid binding proteins;Wuzhishan miniature pig
期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )
ISSN: 1676-5680
年卷期: 2015 年 14 卷 3 期
页码:
收录情况: SCI
摘要: Liver fatty acid binding proteins (L-FABP) are a family of small, highly conserved, cytoplasmic proteins that bind to long-chain fatty acids and other hydrophobic ligands. In this study, a full-length enriched cDNA library was successfully constructed from Wuzhishan miniature pig, and then the L-FABP gene was cloned from this cDNA library and an expression vector (pEGFP-N3-L-FABP) was constructed in vitro. This vector was transfected into hepatocytes to test its function. The results of western blotting analysis demonstrated that the L-FABP gene from our full-length enriched cDNA library regulated downstream genes, including the peroxisome proliferator-activated receptor family in hepatocytes. This study provides a theoretical basis and experimental evidence for the application of L-FABP for the treatment of liver injury.
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