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Bt Proteins Have No Detrimental Effects on Larvae of the Green Lacewing, Chrysopa pallens (Rambur) (Neuroptera: Chrysopidae)

文献类型： 外文期刊

作者： Ali, I. 1 ; Zhang, S. 1 ; Muhammad, M. S. 4 ; Iqbal, M. 5 ; Cui, J. J. 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China

2.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China;^Islamia Univ Bahawalpur, Univ Coll Agr & Environm Sci, Dept Entomol, Baghdad Ul Jadeed Campus, Bahawalpur 63100, Pakistan

3.Islamia Univ Bahawalpur, Univ Coll Agr & Environm Sci, Dept Entomol, Baghdad Ul Jadeed Campus, Bahawalpur 63100, Pakistan

4.Islamia Univ Bahawalpur, Dept Geog, Bahawalpur, Pakistan

5.Islamia Univ Bahawalpur, Dept Biochem & Biotechnol, Bahawalpur, Pakistan

关键词： Bacillus thuringiensis; dietary exposure test; environmental risk assessment; genetically modified crops

期刊名称：NEOTROPICAL ENTOMOLOGY （ 影响因子：1.434； 五年影响因子：1.46 ）

ISSN： 1519-566X

年卷期： 2018 年 47 卷 3 期

页码：

收录情况： SCI

摘要： Biosafety of a genetically modified crop is required to be assessed prior to its commercialization. For this, a suitable artificial diet was developed and used to establish a dietary exposure test for assessing the toxicity of midgut-active Bt insecticidal proteins on Chrysopa pallens (Rambur). Subsequently, this dietary exposure test was used to evaluate the toxicity of the proteins Cry1Ab, Cry1Ac, Cry1Ah, Cry1Ca, Cry1F, Cry2Aa, Cry2Ab, and Vip3Aa on C. pallens larvae. Temporal stability, bioactivity, and the intake of the insecticidal proteins were confirmed by enzyme-linked immunosorbent assay and a sensitive-insect bioassay. The life history characteristics, such as survival, pupation, adult emergence, 7-day larval weight, larval developmental time, and emerged male and female fresh weights remained unaffected, when C. pallens were fed the pure artificial diet (negative control) and the artificial diets containing 200 mu g/g of each purified protein: Cry1Ab, Cry1Ac, Cry1Ah, Cry1Ca, Cry1F, Cry2Aa, Cry2Ab, or Vip3Aa. On the contrary, all of the life history characteristics of C. pallens larvae were adversely affected when fed artificial diet containing boric acid (positive control). The results demonstrate that diets containing the tested concentrations of Cry1Ab, Cry1Ac, Cry1Ah, Cry1Ca, Cry1F, Cry2Aa, Cry2Ab, and Vip3Aa have null effects on C. pallens larvae. The outcome indicates that genetically modified crops expressing the tested Bt proteins are safe for the lacewing, C. pallens.

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