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Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder

文献类型： 外文期刊

作者： Suo, Huayi 1 ; Song, Jia-Le 2 ; Zhou, Yalin 4 ; Liu, Zhenhu 5 ; Yi, Ruokun 3 ; Zhu, Kai 4 ; Xie, Jie 1 ; Zhao, Xin 4 ;

作者机构： 1.Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China

2.Guilin Med Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Guilin 541004, Guangxi, Peoples R China

3.Pusan Natl Univ, Dept Food Sci & Nutr, Pusan 609735, South Korea

4.Chongqing Univ Educ, Dept Biol & Chem Engn, Chongqing 400067, Peoples R China

5.Chinese Acad Agr Sci, Sci & Technol Adm, Beijing 100081, Peoples R China

6.Chongqing Univ Educ, Inst Funct Ecol Food, Chongqing 400067, Peoples R China

关键词： polysaccharide;Larimichthys crocea swim bladder;HCT-116 human colon cancer cells;apoptosis;gene

期刊名称：ONCOLOGY LETTERS （ 影响因子：2.967； 五年影响因子：2.575 ）

ISSN： 1792-1074

年卷期： 2015 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Larimichthys crocea swim bladder is a traditional food and medicine widely used in China. The in vitro anticancer effects of polysaccharide of L. crocea swim bladder (PLCSB) in HCT-116 human colon cancer cells was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. At concentrations ranging between 0 and 800 mu g/ml PLCSB, cancer cell viability was decreased by PLCSB in a concentration-dependent manner. In particular, 400 mu g/ml PLCSB significantly (P<0.05) induced apoptosis, which was demonstrated by 4,6-diamidino-2-phenylindole staining and flow cytometry analysis. To elucidate the mechanisms underlying the anticancer effect of PLCSB in HCT-116 cancer cells, the expression of apoptosis and metastasis-associated genes was analyzed by reverse transcription-polymerase chain reaction and western blot analysis. A total of 400 mu g/ml PLCSB significantly induced apoptosis in HCT-116 cells (P<0.05) via the upregulation Bax, p53, p21, apoptotic protease activating factor 1, caspase-3, -8, and -9, as well as Fas and the downregulation of B-cell lymphoma 2 (Bcl-2), Bcl-extra large and Fos ligand (L). The results of this study demonstrated that PLCSB exhibits an anticancer effect on HCT-116 colon cancer cells, in vitro.

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